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| 丹参总酚酸抑制大鼠肾间质纤维化的初步观察 |
| 尚雁君,黄才国,蒋山好,朱大元,魏善建,焦炳华,李锴男,张建荣 |
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| (第二军医大学基础医学部生物化学和分子生物学教研室,,200433) |
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| 摘要: |
| 目的：观察丹参总酚酸对单侧输尿管梗阻(ＵＵＯ)大鼠肾间质纤维化的影响。方法：将 40只健康、雄性 SD大鼠随机分为４组：分别为UUO组、假手术组（SOR）、阳性对照依那普利（ACEI）组和丹参总酚酸样品(TSA)组,术前分别给UUO组和SOR组灌生理盐水,ACEI组和丹参总酚酸组分别灌胃ACEI\[6 mg/（kg·d）\]和丹参总酚酸\[20 mg/(kg·d)\],连续8 d。术后第 7天分别处死各组大鼠用免疫组织化学方法测定 TGF-β1的表达情况。行H-E和 Masson染色,动态观察肾间质病理学改变。结果：丹参总酚酸组和ACEI组TGF-β1的表达量与模型组(0.22±0.06)比较有明显的下降(P<0.05), 结果分别是1.38±0.26和1.38±0.26。丹参总酚酸能显著减少UUO大鼠肾小管间质TGF-β1的表达,减轻胶原在肾间质的沉积,改善了肾脏病理改变。结论：丹参总酚酸对UUO所致的大鼠肾脏纤维化的形成有明显的抑制作用,其作用可能跟降低TGF-β1的表达有关。 |
| 关键词: 丹参总酚酸 肾间质纤维化 转化生长因子β1? |
| DOI：10.3724/SP.J.1008.2006.01295 |
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| 基金项目: |
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| Total salvianolic acid inhibiting renal interstitial fibrosis in rats? |
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| Abstract: |
| Objective：To study the influence of total salvianolic acid（TSA） on the renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats. Methods: Forty healthy Sprague-Dawley rats were randomly divided into 4 groups：the UUO group, sham-operation group, angiotensin-converting enzyme inhibitor (ACEI)-treated group (positive control), and TSA-treated group. The rats in UUO group and sham-operation group received gastric gavage with normal saline for 8 days before operation; rats in ACEI-treated group and TSA-treated group received ACEI (6 mg/kg by daily gastric gavage for 8 days) and TSA (20 mg/kg by daily gastric gavage for 8 days), respectively. All rats were killed 7 days after operation and the expression of transforming growth factor-β1 (TGF-β1) was detected by immunohistochemical method. The dynamic histological changes of renal interstitial tissues were observed by H-E and Masson staining．Results: TGF-β1 expression in TSA-treated (1.38±0.26) and ACEI-treated (1.38±0.26) groups was significantly lower than that in UUO group(P<0.05). TSA obviously reduced TGF-β1 expression and collagen deposition in the renal interstitial tissues and improved the renal pathological changes in UUO rats. Conclusion: TSA can evidently inhibit UUO-induced renal interstitial fibrosis in rats, which might be related with the down regulation of TGF-β1 expression． |
| Key words: total salvianolic acid renal interstitial fibrosis transforming growth factor-β1 ? |
