| 摘要: |
| 目的：通过对人胸腺素α原(prothymosin-α, ProTα)cDNA测序来分析人胸腺素α原的序列多态性。方法：应用RT-PCR技术从健康人外周血及健康新生儿脐带血中扩增胸腺素α原cDNA，纯化后与克隆载体pMD18-T连接，进行克隆测序，并与标准序列比对，分析其多态性。 结果：序列分析结果表明，克隆的ProTα基因的核苷酸序列并不一致。与已报道的胸腺素α 原基因(NM-002823)进行比较，发现存在2种变异：Ⅰ类变异包括107位单核苷酸突变（A→G）、110～121位和191～205位的核苷酸片段缺失；Ⅱ类变异为306位单核苷酸（G）缺失，多见于年龄60～80岁者。 结论：本研究中ProTα cDNA序列存在2种变异，但并未影响其N-端前28个氨基酸。 |
| 关键词: 胸腺素α原 cDNA 序列分析 |
| DOI：10.3724/SP.J.1008.2008.00092 |
| 投稿时间：2007-02-12 |
| 基金项目: |
|
| Analysis of polymorphism in human cDNA sequence of prothymosin-α |
| GONG Xue-lian, GUO Bao-yu*, GUO Man-ying, Lv Yan |
| (Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective：To analyze the polymorphism in human cDNA sequence of prothymosin-α (ProTα) by sequencing analysis. Methods: The cDNA of human ProTα was amplified from cells of peripheral blood and cord blood by RT-PCR．The product of RT-PCR was purified and linked with vector pMD18-T. After cloning and sequencing, the sequence of ProTα cDNA was compared with the standard sequence to analyze the polymorphism in the ProTα cDNA sequence. Results: The cloned ProTα cDNA sequence was different from that of the standard. We found 2 kinds of variations: (1) The nucleotide in 107 position was varied and the nucleotides in 110-121 and 191-205 positions were deleted; (2) The nucleotide in 306 position was deleted, mainly in the 60-80 years old group. Conclusion: We have identified 2 kinds of variations in human ProTα cDNA, but the first 28 amino acid in the N-terminal of cDNA of human ProTα are not involved therefore the variations do not affect the function of human ProTα. |
| Key words: prothymosin-α cDNA sequence analysis |
