| 摘要: |
| 目的：建立人肺鳞癌细胞系，探讨其生物学特性。方法：取人肺鳞癌新鲜手术标本，经组织块体外培养并克隆建系，命名为CHLH-1。采用光镜、电镜、染色体核型分析及异种移植瘤实验对细胞系生物学特性进行观察。结果：原位肿瘤、细胞系及移植瘤标本经光镜、电镜证实该细胞系具有鳞状上皮性恶性细胞特征，并观察了其体外生长曲线、倍增时间和分裂指数；染色体核型分析众数为60~68，为亚三倍体；裸鼠皮下异种移植形成移植瘤。结论：根据细胞系特征显示该细胞系是一新建的肺鳞癌细胞系。 |
| 关键词: 鳞癌 细胞系 肺 |
| DOI：10.3724/SP.J.1008.2007.01375 |
| 投稿时间：2007-03-09 |
| 基金项目:上海市科委基金 (04JC14006）. |
|
| Establishment of human lung squamous carcinoma cell line CHLH-1 |
| LIU Hong-cheng，HUANG Sheng-dong，GONG De-jun，LIU Xiao-hong，YUAN Yang，XU Zhi-yun* |
| (Department of Cardiothoracic Surgery，Changhai Hospital, Second Military Medical University，Shanghai 200433, China) |
| Abstract: |
| Objective：To establish a human lung squamous carcinoma cell line and to study its biological characteristics．Methods: Lung squamous carcinoma specimens were freshly resected during operation; the tissues were incubated in vitro and the cell line was named CHLH-1. The biological characteristics of the cells were studied by light microscopy, electron microscopy, chromosome analysis and transplantation experiment. Results: Cells from the specimens of the primary tumor, the CHLC-1 cell line and the cells from transplanted tumor possessed the characteristics of malignant squamous epithelium under light and electron microscope. The cell growth curve, doubling time and mitotic index were also observed in vitro. Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 60-68 per cell. Tumor nodes were observed under the skin of nude mice by heterogenic transplantation. Conclusion: The characteristics of the established cell line suggest that it is a newly established human squamous carcinoma cell line． |
| Key words: squamous carcinoma cell line lung |
