| 摘要: |
| 目的：研究人类非小细胞肺癌（NSCLC）组织中ATP合酶F1ATPaseα的表达，并探讨其临床意义。方法：（1）以EnVision免疫组织化学方法检测38例NSCLC配对标本、7例肺良性肿瘤与肺炎性病变标本的F1ATPaseα表达情况。（2）应用RTPCR方法检测12例NSCLC组织、癌旁肺组织中F1ATPaseα的mRNA表达。（3）用免疫荧光法检测F1ATPaseα在正常支气管上皮细胞与A549细胞胞膜中的表达。 结果：(1) F1ATPaseα在肺癌组织呈中高度表达者36例（36/38），低度表达2例；癌旁正常配对组织成呈中高度表达11例（11/38），低度表达27例;7例肺良性病变组织全部低度表达。肺腺癌F1ATPaseα的高表达显著高于肺鳞癌(11/16 vs 5/20，P<0.05)。F1ATPaseα高表达率在肿瘤不同部位、分化程度、大小、淋巴结转移及临床分期之间差异无统计学意义。(2) 12例肺癌组织中F1ATPaseα mRNA相对表达量为0.54±0.19，显著高于配对的癌旁肺组织（0.31±0.12，P<0.01)。(3) 肺癌A549细胞胞膜上有颗粒状分布的F1ATPaseα，正常支气管上皮细胞未检测到。结论：（1）F1ATPaseα在NSCLC中有相对较高的表达水平。（2）F1ATPaseα表达于非小细胞肺癌A549细胞株的细胞膜上，提示有作为靶向药物治疗分子靶点的潜力。 |
| 关键词: ATP合成酶复合物 癌，非小细胞肺 免疫组织化学；免疫荧光化学 |
| DOI：10.3724/SP.J.1008.2007.01219 |
| 投稿时间：2007-03-29 |
| 基金项目: |
|
| Expression of F1ATPase-α in non small cell lung cancer and its clinical significance |
| ZHU Hai-mo,LI Min-yu, SHI Ping, ZHOU Jun-ping, LOU Guo-liang* |
| (Department of Special Clinic, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective：To study the expression of F1ATPaseα in nonsmall cell lung cancer and its clinical significance. Methods: (1)The expression level of F1ATPaseα was examined by immunohistochemical Envision method in 38 NSCLC samples and the adjacent normal tissues, 7 benign tumors and chronic pneumonia samples. (2)F1ATPaseα mRNA expression were detected in 12 fresh samples of NSCLC and the adjacent normal tissues by PTPCR.(3)The expression of F1ATPaseα on the pericellular membrane of A549 cells was observed by immunofluorescence. Results: (1)Immunohistochemistry analysis showed median and higher expression of F1ATPaseα in 36 of NSCLC specimens and 11 adjacent normal tissues; lower expression was detected in all the benign samples.The overexpression of F1ATPaseα in lung adenocarcinoma tissues were significantly higher than that in the lung squamous cancer (P<0.05). The expression of F1ATPaseα in NSCLC was not associated with the histology type, location, differentiation degree, size, invasion and metastasis. (2) The relative level of ECTOF1ATPaseα was 0.31±0.12 in the adjacent normal tissues and 0.54±0.19 in NSCLC tissues (P<0.01). (3)The conspicuous positive expression of F1ATPaseα on the pericellular membrane of A549 cells was not observed on normal bronchial epithelial cells.Conclusion: （1）NSCLC has a higher expression of F1ATPaseα and the expression is on the pericellular membrane of A549 cells, which may provide a new target for molecular therapy of NSCLC. |
| Key words: ATP synthetase complexes carcinoma,non-small-cell-lung immunohistochemistry immunofluorescence |
