| 摘要: |
| 目的:研究天冬总皂苷灌胃给药对麻醉犬脑血流量（CBF）及脑血管阻力（CVR）的影响。方法:杂种犬30只,体质量（11±1.5） kg,雌雄兼用,随即分为5组（每组6只）:阴性对照组（生理盐水5 ml/kg,灌胃给药）、阳性对照组（尼莫地平注射液 300 μg/kg,iv）、天冬总皂苷按10、30、60 mg/kg分低中高3个剂量组,灌胃给药。犬用戊巴比妥钠30 mg/kg静脉注射麻醉,手术暴露右侧颈总动脉,用MFV-3200型电磁流量计测定CBF和CVR；MPA3000生物电放大器记录血压（SBP、DBP）和心率（HR）等指标。结果:与阴性对照组比较，天冬总皂苷30、60 mg/kg组于给药后5 min即能明显增加CBF并一直持续到120 min (P<0.01)，CBF随剂量增加而增加，显示出有较好的量效关系；天冬总皂苷30、60 mg/kg组用药后CVR虽较用药前降低，但大部分时间点未达到统计学显著意义；天冬总皂苷10 mg/kg组CBF、CVR无明显变化。结论:天冬总皂苷灌胃给药有显著增加麻醉犬CBF的作用。 |
| 关键词: 天冬总皂苷 麻醉 脑血流 脑血管阻力 血压 心率 |
| DOI：10.3724/SP.J.1008.2008.00431 |
| 投稿时间：2007-05-16修订日期：2008-02-29 |
| 基金项目:国家自然科学基金（20472113）. |
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| Influence of total saponins from Asparagus cochinchinensis on cerebral blood flow and vascular resistance in anesthetized dogs |
| LIU Jian-guo,CHEN Hai-sheng*,XU Cong-li,SHEN Yang |
| (Department of Natural Medicinal Chemistry,School of Pharmacy,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To study the effect of total saponins on cerebral blood flow and vascular resistance in anesthetized dogs.Methods: Thirty hybrid dogs in either sex,with a body weight of (11±1.5) kg,were evenly randomized into 5 groups:negative control group(saline 5 ml/kg,ig),positive control (nimodipine 300 μg/kg,iv),and 3 groups treated with total saponins (low-dose group \[10 mg/kg,ig\],middle-dose group\[30 mg/kg,ig\],and high-dose group\[60 mg/kg,ig\]).The dogs were anesthetized with intravenous pentobarbital sodium (30 mg/kg).The right common carotid artery was exposed to measure the cerebral blood flow,cerebral vascular resistance,blood pressure and heart rate using the MFV-3200 electromagnetic flow meter and MPA-3000 bioelectricity signal-amplifier.Results: Compared with negative control, cerebral blood flow was significantly increased in animals treated with asparagus root saponins（30 and 60 mg/kg,ig）during 5 and 120 min after drug administration (P<0.01). No significant effect on cerebral blood flow and vascular resistance was found in animals treated with asparagus root saponins（10 mg/kg）. Conclusion: Asparagus root saponins can increase cerebral blood flow in anesthetized dogs.
\[KEY WORDS\]total saponins from Asparagus cochinchinensis; anesthesia; cerebral blood flow; vascular resistance; blood pressure; heart rate |
| Key words: total saponins from Asparagus cochinchinensis anesthesia cerebral blood flow vascular resistance blood pressure heart rate |
