| 摘要: |
| 目的：观察设计合成的两种融合肽对小鼠烫伤的疗效。方法：针对烫伤经历炎症反应、感染、而后细胞增殖修复的特点，选择相应的氨基酸序列人工合成两种融合肽M-Tα1 和 M-T508，将其应用于深Ⅱ度烫伤的小鼠模型上，观察愈合情况。同时，为了在微观领域证实愈合情况，选择了6个检测指标，包括：利用免疫组化方法检测烫伤皮肤组织中Ⅰ型胶原、血管内皮生长因子（VEGF）、增殖细胞核抗原（PCNA）和细胞角蛋白（CK）的表达；用酶联免疫吸附试验（ELISA）检测烫伤小鼠血清中TNF-α的含量变化和伊文思蓝（Evans blue）染色检测烫伤皮肤组织中血管通透性的改变。结果：两组用药组比对照组创伤愈合时间提前1周左右；免疫组化结果显示两组用药组第10天Ⅰ型胶原、VEGF、PCNA和CK都呈阳性表达，而同时相的对照组各个指标则是阴性结果。烫伤组织TNF-α的ELISA检测和血管通透性的Evans blue检测则印证了我们所合成的融合肽有抗炎、抗渗作用。结论：设计合成的两种融合肽对烫伤小鼠均有明显加快愈合的作用。 |
| 关键词: 融合肽 电烧伤 伤口愈合 免疫组织化学 |
| DOI：10.3724/SP.J.1008.2008.00259 |
| 投稿时间：2007-06-06修订日期：2007-12-14 |
| 基金项目: |
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| Two fusion peptides promote healing of scalded mice |
| FANG Chen,GUO Bao-yu* |
| (Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective：To study the therapeutic effect of 2 artificially synthesized fusion peptides on scalded mice. Methods: Two new fusion peptides, M-T508 and M-Tα1, were synthesized based on amino acid sequences selected according to the characteristics of scald (including 3 stages: inflammation, infection and repair by cell proliferation). The 2 peptides were applied for second degree scald burn in mice and the healing of the scald burn was observed. The expression ofⅠcollagen, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and cytokeratin (CK) was detected by immunohistochemical staining. Serum TNF-α concentration was determined by ELISA in the animals and the blood vessel permeability was analyzed by Evans blue staining. Results: The healing periods of scald burns in the 2 peptide groups were 1 week earlier than that in the control group. Immunohistochemical results showed that the expression of collagenⅠ, VEGF, PCNA and CK was all positive in the 2 peptide groups 10 days after scald and negative in the control group. Examination of blood vessel permeability and serum TNF-α concentration in the M-Tα1 and M-T508 groups showed that the 2 fusion peptides had anti-inflammation and anti-infiltration effects in scalded mouse. Conclusion: The synthesized fusion peptide M-Tα1 and M-T508 can promote the healing of scald burns in mice. |
| Key words: fusion peptides electric burns would healing immunohistochemistry |
