| 摘要: |
| 目的：观察聚乙烯亚胺（polyethylenimine,PEI）作为基因载体介导BMP-7基因体内转染促进骨折愈合的效果。方法：20月龄雄性SD大鼠随机分为3组：BMP-7+PEI治疗组、生理盐水对照组及BMP-7对照组（n=20）。所有大鼠建立右股骨干骨折模型，BMP-7+PEI治疗组于骨折断端经皮注射pcDNA3.1-BMP-7/PEI混合试剂，生理盐水及BMP-7对照组分别注射等量生理盐水及pcDNA3.1-BMP-7。治疗后2、4、8周观察X线片、H-E染色、Ⅰ型胶原免疫组化染色、生物力学和骨密度检测结果，判断骨折愈合情况。结果：X线及H-E染色结果表明，3组老年大鼠骨折后第8周均未获得愈合，但BMP-7+PEI治疗组骨痂生长情况优于生理盐水及BMP-7对照组，断端间出现部分骨性连接。Ⅰ型胶原染色表明，与生理盐水及BMP-7对照组相比，BMP-7+PEI治疗组Ⅰ型胶原染色深、分布范围较大。抗弯曲强度和骨密度检测结果表明，BMP-7+PEI治疗组各项指标均优于对照组，差异有统计学意义（P<0.05或0.01）。结论：PEI介导BMP-7基因体内转染可有效促进老年大鼠骨折愈合。 |
| 关键词: 聚乙烯亚胺；BMP-7；转染；骨折 老年大鼠 |
| DOI：10.3724/SP.J.1008.2008.00514 |
| 投稿时间：2007-06-11 |
| 基金项目: |
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| Polyethylenimine-mediated BMP-7 gene transfection promotes fracture healing in elderly rats |
| HAN Qing-lin1,GOU San-huai1*,WANG Liang-zhe2,KANG Jian1,WANG Qi1 |
| (1. Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;2. Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200003) |
| Abstract: |
| Objective：To observe polyethylenimine-mediated BMP-7 gene transfection in promoting fracture healing in elderly rats. Methods：Male SD rats (20-month-old) were randomly divided into 3 groups: group A received BMP-7 gene therapy mediated by polyehtylenimine, group B was treated with normal saline and group C was treated with BMP-7 plasmid without polyethylenimine. Right femoral shaft fracture model was established in all rats. Animals in group A received transdermal injection of pcDNA3.1-BMP-7/PEI; those in group B and C received the same volume of normal saline and pcDNA3.1-BMP-7, respectively. X-ray photography, histological observation (H-E staining), immunohistochemical staining of collagen type Ⅰ, biomechanical and bone mineral density test were employed to assess the healing of fracture 2, 4, and 8 weeks after treatment. Results: The results of X ray and H-E staining showed no fracture healing in the 3 groups during the 8th week after fracture; however, the growth of coloboma in group A was better than that of the other 2 groups, with partial bone union between the fracture ends. Staining of collagen type Ⅰ showed deeper, wider staining in group A compared with the other 2 group. Anti-bending intensity and bone mineral density tests showed that the parameters in group A were better than those of the other 2 groups (P<0.05 or 0.01). Conclusion: Polyethylenimine-mediated hBMP-7 gene transfer can effectively promote fracture healing in elderly rats. |
| Key words: polyethylenimine BMP-7 transfection fracture old rats |
