| 摘要: |
| 目的：检测可卡因-苯丙胺调节转录肽(CART)蛋白疫苗在吗啡镇痛及其耐受中的作用。方法：利用基因克隆技术构建pGEX-4T3-CART55-102表达质粒，通过谷胱甘肽S转移酶(GST)亲和层析法纯化出GST-CART蛋白。 实验分为6组:空白对照组，生理盐水组，GST+ 弗氏佐剂组，CART蛋白疫苗5 μg组、10 μg组和20 μg组，免疫两次后热板法对各组进行痛反应检测。皮下注射6 mg/kg吗啡进行镇痛效应检测，计算最大镇痛效应百分率（MPE%）。随后建立吗啡耐受模型，并于末次给药12 h后皮下注射6 mg/kg吗啡进行耐受效应检测。结果：CART蛋白疫苗本身对基础痛阈没有影响（P>0.05）。10 μg CART蛋白疫苗组显著降低了吗啡镇痛效应（P<0.05）。对比生理盐水组，疫苗组显示出抗耐受的潜力，其中10 μg组 MPE%增高具有显著性差异（P<0.05）。结论：CART蛋白疫苗本身对痛反应没有影响，但削弱了吗啡的镇痛效应，同时对吗啡镇痛耐受具有拮抗作用。 |
| 关键词: 可卡因-苯丙胺调节转录肽蛋白；疫苗 吗啡 镇痛 药物耐受性 |
| DOI：10.3724/SP.J.1008.2008.00349 |
| 投稿时间：2007-07-31修订日期：2008-03-24 |
| 基金项目:国家重点基础研究规划（“973”计划）（2003CB515400）；国家自然科学基金（30371642）. |
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| Effect of CART55-102 protein vaccine on morphine analgesia and tolerance |
| SONG Juan,GUO Wei,CHAI Jing-rui,YOU Zhen-dong*,LU Chang-lin |
| (Department of Neurobiology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective：To study the effect of cocaine- and amphetamine-regulated transcript (CART) protein vaccine on morphine analgesia and tolerance.Methods: The expression plasmid pGEX-4T3-CART was constructed by gene cloning.The CART protein was purified by glutathione s-transferase(GST)-affinity chromatography.The experiment included 6 groups:blank control,normal saline (NS),GST+Freund’s adjuvant,and CART protein vaccine (5 μg,10 μg and 20 μg) groups.After immunization for twice,all groups were tested in hot plate.Morphine analgesia effect was evaluated through s.c. injection with 6 mg/kg morphine solution,calculated by MPE%.Then morphine tolerance model was established,and the tolerance to morphine was tested by s.c. injection with 6 mg/kg morphine 12 h after the last injection.Results: CART vaccine itself had no pronounced effect on the pain threshold (P>0.05).CART vaccine at 10 μg significantly depressed the analgesic effect of morphine analgesia (P<0.05).Compared with NS group,vaccine groups showed a potential antagonizing tolerance effect,especially in the 10 μg group,with the MPE% significantly increased(P<0.05).Conclusion: CART vaccine itself has no influence on the pain response; however,it can impair the analgesia effect of morphine and can antagonize the analgesia tolerance to morphine. |
| Key words: cocaine- and amphetamine-regulated transcript protein vaccine morphine analgesia drug tolerance |
