| 摘要: |
| 目的：分析乙型肝炎病毒(HBV)前C区1896位基因突变与HBV基因型、HBV复制以及患者肝功能之间的关系。方法：对60例HBV DNA阳性患者进行荧光扩增检测前C区1896变异、HBV DNA基因分型、HBV DNA定量检测,酶免微粒子化学发光法检测血清HBeAg及HBeAb,全自动生化仪检测肝功能指标。结果：前C区1896变异者ALT水平为（109±111） U/L,比野生型者显著上升(P＜0.05)；1896位点变异与HBeAg、HBV基因型及HBV DNA水平无关；乙型肝炎患者HBV DNA水平C型显著高于B型(P＜0.05)。结论：HBV DNA前C区1896变异感染可能与肝细胞受损加重有关；基因型C型HBV DNA水平显著高于B型，提示C型病毒复制更为活跃。 |
| 关键词: 点突变 基因型 肝炎病毒,乙型 |
| DOI：10.3724/SP.J.1008.2007.01302 |
| 投稿时间：2007-08-05修订日期：2007-10-19 |
| 基金项目:国家自然科学基金(30571774). |
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| Analysis of HBV precore 1896 site mutation and its HBV genotype and other laboratory features |
| JI Qiang，GAO Chun-fang*，ZHAO Yun-peng，LU Ying，WANG Ai-hua,CHEN Si-jia |
| (Department of Laboratory Medicine,Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438,China) |
| Abstract: |
| Objective：To analyze the relationship between hepatitis B virus (HBV) gene mutation at 1896 in precore region with genotype and replication of HBV and the liver function of patients. Methods：HBV precore 1896 site mutation,the genotype of HBV and serum content of HBV DNA were determined by PCR in 60 patients positive of HBV DNA. Chemiluminescence miacropaticle immunoassay (CMIA) was used for detection of serum HBeAg and HBeAb. Liver function parameters were obtained by routine biochemistry method. Results：The alanine aminotransferase (ALT) level in HBV with 1896 site mutation was significantly higher than that in the wildtype virus.Site mutation at 1896 had no correlation with HBeAg, HBV genotype and HBV DNA content. HBV DNA content in patient with genotype C was significantly higher than that with genotype B(P＜0.05).Conclusion：Mutation at 1896 in precore region of HBV gene is related to aggravation of hepatocyte injury. The serum content of HBV DNA is higher in genotype C than that in genotype B, indicating genotype C HBV has more active replication activity than genotype B. |
| Key words: point mutation genotype hepatitis B virus |
