| 摘要: |
| 目的：探讨乙肝病毒（HBV）前S1（Pre-S1）抗原与e抗原（HBeAg）、HBV DNA含量的相关性，以评价Pre-S1抗原检测HBV复制的临床应用价值。方法：临床采集乙肝表面抗原阳性标本363例，以ELISA法检测Pre-S1抗原,时间分辨免疫荧光法检测HBeAg,实时荧光定量PCR（FQ-PCR）法检测HBV DNA含量,并对Pre-S1抗原、HBeAg和HBV DNA检测结果进行相关性分析。结果：Pre-S1抗原与HBeAg值之间存在关联性（χ2=94.4,P<0.01）,关联系数为0.45；Pre-S1抗原与HBV DNA亦有关联(χ2=198.58,P<0.01)，关联系数为0.59。Pre-S1抗原阳性率随HBV DNA含量的增加而增加。当HBV DNA拷贝数的对数值>3时，Pre-S1抗原的诊断敏感度84.5%，特异性91.2% ，准确性87.0％，阳性预测值94.1%，阳性似然比9.6，优势比56.8；HBeAg诊断敏感度50.4%，特异性94.9%，准确性67.2％，阳性预测值94.2%，阳性似然比9.8，优势比18.9。结论：Pre-S1抗原与HBV DNA具有相关性，Pre-S1抗原较HBeAg能更敏感、更准确地反映HBV复制情况，三者联合检测互相补充，更有助于临床疾病的诊治。 |
| 关键词: 肝炎病毒，乙型 前S1抗原 e抗原 DNA |
| DOI：10.3724/SP.J.1008.2007.01306 |
| 投稿时间：2007-09-17修订日期：2007-10-10 |
| 基金项目: |
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| Correlation analysis between HBV Pre-S1 antigen with HBeAg and HBV DNA |
| HOU Xiao-jing, LIANG Yan, HE Feng-chun, CHEN Jie，CUI Yan-fang，ZHONG Ren-qian, WANG Hao* |
| (Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China) |
| Abstract: |
| Objective：To study the correlation between HBV Pre-S1 antigen, HBeAg levels and HBV DNA copies, so as to assess the clinical value of Pre-S1 in detection of HBV replication. Methods: A total of 363 HBsAg-positive samples were collected. The levels of Pre-S1 antigen, HBeAg and HBV DNA copies were determined by ELISA, time-resolved immuno-fluorescent method and real-time fluorescent quantitative PCR(FQ-PCR),respectively. The correlation between the determination results was analyzed. Results: Pre-S1 antigen level was correlated with the level of HBeAg (χ2=94.4,P<0.01), with the coefficient being 0.45; Pre-S1 antigen level was also correlated with HBV DNA(χ2=198.58, P<0.01), with the coefficient being 0.59. The positive rate of Pre-S1 increased with HBV DNA copies. When logarithm of HBV DNA copies was more than 3, the sensitivity, specificity, accuracy, positive predictive value(PPV), positive likelihood ratio(PLR) and odds ratio(OR) of Pre-S1 were 84.5%,91.2%,87.0％,94.1%,9.6，and 56.8 respectively；the numbers of HBeAg were 50.4%,94.9%，67.2％，94.2%,9.8 and 18.9, respectively. Conclusion: Pre-S1 antigen is correlated with HBV DNA and is more sensitive and accurate than HBeAg in reflecting HBV replication. The above 3 parameters can complement each other and the combination of them is more accurate in clinical diagnosis. |
| Key words: hepatitis B virus pre-S1 antigen e antigen DNA |
