| 摘要: |
| 目的:用海螵蛸中提取的多糖盐洗组分(CBP-s)预处理小鼠后,用无水乙醇灌胃处理,观察小鼠胃黏膜的损伤程度,探究其保护胃黏膜的机制。方法:用CBP-s分别预处理小鼠3 d和5 d,再用无水乙醇诱导其胃黏膜损伤,测定溃疡指数、胃内容物pH值,以及胃黏膜的一氧化氮(NO)、谷胱甘肽(GSH)、丙二醛(MDA)和过氧化物歧化酶(SOD)等指标。结果:100、200和400 mg/kg CBP-s预处理3 d和5 d的小鼠溃疡指数下降；100、200和400 mg/kg CBP-s预处理3 d和5 d,胃内容物pH值分别是3.09±0.20、3.43±0.23、3.32±0.15、3.34±0.25、3.38±0.15、3.68±0.24；400 mg/kg CBP-s预处理3 d和5 d,胃黏膜的MDA含量较造模组都有显著下降\[(125.41±28.79) vs (145.80±26.13);(153.88±21.19) vs (127.14±5.68) nmol/g,P<0.05\]；除了100 mg/kg预处理3 d组,各剂量组均能显著提高GSH和NO的含量\[GSH:(282.93±32.33),(293.57±20.76),(260.79±39.23),(253.71±21.11),(359.38±10.89) mg/g,P<0.05\]；NO:(23.40±4.41),(25.83±4.89),(21.60±3.05),(22.75±2.44) μmol/g,P<0.05\]；各组的SOD活性均有显著上升\[(30.61±6.37),(45.35±1.90),(47.12±4.68),(36.48±5.64),(41.38±8.23),(39.42±8.76) U/mg,P<0.05\]。结论:CBP-s对乙醇诱导的小鼠胃黏膜具有细胞保护作用。 |
| 关键词: 胃溃疡 胃黏膜 海螵蛸 多糖 溃疡指数 细胞保护 |
| DOI：10.3724/SP.J.1008.2008.01328 |
| 投稿时间：2007-09-18修订日期：2008-09-22 |
| 基金项目: |
|
| Protective effect of Cuttlebone polysaccharides on gastric mucosa in mice |
| GUO Yi-feng,ZHOU Wen-li,ZHANG Jian-peng,LIU Jun-hua,FENG Wei-hua*,JIAO Bing-hua* |
| (Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To observe the protective effect of cuttlebone polysaccharides (CBP-s) on gastric mucosa lesions induced by absolute ethanol in mice,and to study the related mechanism.Methods:Mice were pretreated by CBP-s for 3 or 5 days and then were administered with absolute ethanol orally to induce gastric mucosa lesions.Then the ulcer index,pH value of gastric contents,the levels of nitric oxide (NO),malondialdehyde (MDA),reduced glutathione (GSH),and the activity of SOD in the gastric mucosa were studied.Results:Mice gastric ulcer indices decreased after pretreated with 100 mg/kg,200 mg/kg,and 400 mg/kg CBP-s for 3 or 5 days.The pH values of gastric contents after 3 day-pretreatment were 3.09±0.20,3.32±0.15,3.38±0.15,and after 5 day-pretreatment were 3.43±0.23,3.34±0.25,3.68±0.24,respectively.MDA contents of gastric mucosa after 3 day-pretreatment with 400 mg/kg CBP-s and after 5 day-pretreatment with all 3 concentrations were (125.41±28.79),(145.80±26.13),(153.88±21.19),and (127.14±5.68) nmol/g,respectively,all significantly lower than that of the model group (P<0.05).GSH and NO contents were significantly increased in all but 100 mg/kg for 3 days group (GSH:\[282.93±32.33\],\[293.57±20.76\],\[260.79±39.23\],\[253.71±21.11\],\[359.38±10.89\] mg/g,P<0.05),(NO:\[23.40±4.41\],\[25.83±4.89\],\[21.60±3.05\],and \[22.75±2.44\] μmol/g,P<0.05).The activities of SOD were significantly increased in all groups (\[30.61±6.37\],\[45.35±1.90\],\[47.12±4.68\],\[36.48±5.64\],\[41.38±8.23\],and \[39.42±8.76\] U/mg,P<0.05).Conclusion:Our results indicate that CBP-s can protect ethanol-induced gastric mucosa from injury in mice. |
| Key words: stomach ulcer gastric mucosa os sepiae polysaccharide ulcer index cytoprotection |
