| 摘要: |
| 目的：制备稳定性高、粒径小的脑源性神经营养因子（BDNF）缓释注射纳米粒，并评价其释药过程。方法：采用乳酸-羟基乙酸共聚物（PLGA）为载体材料，复乳化溶剂挥干法制备载有BDNF的PLGA纳米粒。优化纳米粒处方和制备工艺，观察纳米粒的形态、大小和粒径分布，评价其回收率、精密度、重复性、包封率以及体外释药特性。结果：优选处方选择理论载药量1%、聚合物浓度3.3 mg/ml、超声时间为40 s，甘露醇为支架剂。BDNF纳米粒呈圆形，大小均匀，平均粒径为156.7 nm。制备的纳米粒回收率、精密度、重复性和包封率较高，缓慢溶蚀释放为其主释药过程，时间达30 d。结论：成功制备的BDNF缓释注射纳米粒具有稳定性好、包封率高的特点。 |
| 关键词: 脑源性神经营养因子 缓释制剂 纳米粒 |
| DOI：10.3724/SP.J.1008.2008.00538 |
| 投稿时间：2007-10-08修订日期：2008-01-25 |
| 基金项目:国家自然科学基金（30300359）,上海市自然科学基金(07JC14072). |
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| Preparation of injectable sustained-release nanoparticles carrying brain-derived neurotrophic factor and evaluation of their drug releasing characteristics |
| SHI Guo-dong1,JIA Lian-shun1,YUAN Wen1,SHI Jian-gang1*,TAN Jun-ming1,CHU Cang2 |
| (1.Department of Orthopedics,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;2.Department of Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective：To prepare stable, small-sized injectable sustained-release nanoparticles harboring brain-derived neurotrophic factor (BDNF) and to evaluate its drug releasing process.Methods: The nanoparticles were prepared using poly(D,L-lactic-co-glycolic acid) (PLGA) as the carrier by w/o/w double emulsion-solvent evaporation method.The formula and technique were optimized; the shape,size and the distribution of the diameters of the particles were observed; and recovery rate,precision,repeatability,encapsulation efficiency,and drug releasing characteristics were assessed.Results: With the optimized formula,the drug loading rate was 1%,the polymer concentration was 3.3 mg/ml, and the ultrasound time was 40 s; mannitol was used as the supporting agent.BDNF nanoparticles were round,homogenous in size,with a mean diameter of 156.7 nm.The prepared particles had high recovery rate,precision,repeatability,and encapsulation efficiency.The drug release was characterized by slow corrosion and the process lasted for 30 days.Conclusion: We have successfully prepared slow-release nanoparticles harboring BDNF,which are stable and have high encapsulation efficiency. |
| Key words: brain-derived neurotrophic factor sustained-release preparations nanoparticles |
