| 摘要: |
| 目的：通过高氧诱导C57BL／6J小鼠视网膜新生血管动物模型，观察CD105在视网膜新生血管中的表达。方法：取出生后7d的C57BL／6J小鼠40只，随机分为正常对照组和高氧组，每组20只。采用高氧诱导视网膜新生血管建立动物模型，H—E染色观察小鼠视网膜新生血管的情况，免疫组化方法观察CD105在小鼠视网膜中的表达。结果：H—E染色结果表明，小鼠视网膜新生血管模型建立成功。高氧组小鼠CD105显著表达，积分光密度为（9985．63±1016．28）／鼠，阳性染色面积为（14246．61±6052．29）μm^2／鼠，而正常对照组仅有微弱表达[积分光密度为（1625．36±638．44）／鼠，阳性染色面积为（3619．31±1760．03）μm^2／鼠]，两组相比差异具有统计学意义（P〈0．01）。结论：CD105在视网膜新生血管形成中可能有一定作用，其检测对于视网膜新生血管的定性、定量评估及治疗具有一定临床意义。 |
| 关键词: CD105 视网膜疾病 视网膜新生血管化 |
| DOI：10.3724/SP.J.1008.2007.00166 |
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| 基金项目: |
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| Expression of CD105 in oxygen-induced retinal neovascularization models in mice |
| LI Juan , LI Li ,TANG Yong-qiang , WANG Qiang |
| (1. Department of Ophthalmology, The Second Affiliated Hospital of Chongqing University of Medical Sciences,Chongqing 400010, China; 2. Department of Hyperbaric Oxygen, Daping Hospital, Third Military Medical University, Chongqing 400042) |
| Abstract: |
| Objective： To investigate the expression of CD105 in oxygen-induced retinopathy （OIR） in C57BL/6J mice. Methods： Forty 7-day old C57BL/6J mice were evenly randomized into normal control group and hyperbaric oxygen group. The OIR model was induced with hyperbaric oxygen; the retinal neovascularization was observed by H-E staining and the expression of CD105 in OIR was observed by immunohistochemistry staining. Results： H-E staining indicated that the retinal neovascularization model was successfully estabished. CD105 was strongly expressed in the hyperbaric oxygen group, with the integral photodensity of angiogenesis being 9 985.63± 1 016.28 per mouse and the positive staining area being （14 246.61 ± 6 052.29） μm^2 per mouse; CD105 was weakly expressed in the control group with the integral photodensity of angiogenesis being 1 625.36±638.44 per mouse and the positive staining area being （3 619.31±1 760.03） μm^2 per mouse; the differences between the 2 groups were statistically significant （P 〈 0. 01 ）. Conclusion： CD105 may play a role in the retinal neovascularization; detection of CD105 might be valuable in evaluating retinal neovascularization and the subsequent treatment. |
| Key words: CD105 retinal diseases retinal neovascularization |
