| 摘要: |
| 目的：探讨C型利尿钠肽（CNP）对晚期糖基化终末产物（AGEs）抑制的利尿钠肽B受体（NPR—B）及受体后信号的影响。方法：体外培养人肾小球系膜细胞，分别用免疫荧光染色法、酶联免疫吸附分析法和流式细胞术观察CNP和AGEs作用下的NPR-B免疫荧光表达、细胞内cGMP水平和NF-κBp65活性亚基的表达。结果：CNP可以显著上调NPR—B的荧光表达、显著升高AGEs抑制的细胞内cGMP浓度（P〈0．001）及抑制AGEs刺激的NF-κBp65活性亚基的表达。结论：CNP能够调节AGEs影响的NPR—B／cGMP信号及NF-κB活性，并且可能通过NPR—B／cGMP途径发挥抑制NF-κB活性的作用。[著者文摘] |
| 关键词: 利尿钠肽,C型 肾小球系膜细胞 糖基化终末产物 |
| DOI：10.3724/SP.J.1008.2007.00758 |
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| 基金项目:国家重点基础研究发展计划（973计划）（2005CB523304） |
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| Influence of C-type natriuretic peptide on expression of natriuretic peptide B receptor suppressed by advanced glycation end products and on post-receptor signal regulation |
| QU Wei, ZOU Junjie, SHI Yong-quan, ZHANG Chun-yang, LIU Zhi-min |
| (Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China) |
| Abstract: |
| Objective：To investigate the influence of C-type natriuretic peptide（CNP） on the expression of natriuretic peptide B receptor（NPR B） and its cGMP signal induced by advanced glycation end products（AGEs） in human mesangial cells（HMC）. Methods： HMCs were cultured in vitro. The expression of NPRB, intracellular concentration of cGMP and level of NF-κB p65 in HMC were measured by immunofluorescent staining, enzyme linked immunosorbem assay（ELISA） and flow cytometry, respectively. Results： CNP significantly upregulated the immunofluorescent intensity of NPR-B and the concentration of cGMP inhibited by AGEs（P〈0.001） ;CNP also inhibited the fluorescent intensity of NF-κB p65 stimulated by AGEs. Conclusion： CNP can regulate the expression of NPR-B/ cGMP and activity of NF-κB induced by AGEs through a NPR- B/cGMP dependent manner.[著者文摘] |
| Key words: natriuretic peptide,C-type mesangial cell glycosylation end products |
