| 摘要: |
| 目的:对芫花条的抗炎活性成分进行研究。方法:采用药理活性追踪下的化学成分分离手段,从芫花条中发现抗炎活性成分。药理实验采用二甲苯致小鼠耳肿胀药理模型。运用硅胶、Sephadex LH-20柱色谱等方法对芫花乙醇提取物的石油醚及氯仿萃取部分进行化学成分研究,并利用UV、IR、1HNMR、13C NMR、MS等光谱技术鉴定其化学结构。结果:从芫花条中分离得到了19个化合物,分别鉴定为十六烷酸1-甘油酯(1)、β-谷甾醇(2)、咖啡酸正二十烷酯(3)、咖啡酸正二十二烷酯(4)、咖啡酸正十八烷酯(5)、胡萝卜苷(6)、芫花素(7)、木犀草素(8)、(+)落叶松脂醇(9)、芹菜素(10)、山柰酚(11)、瑞香黄烷素B(12)、染料木素(13)、二氢山柰酚(14)、对羟基苯甲酸(15)、槲皮素(16)、丁香苷(17)、丁香醛(18)、对羟基苯甲酸乙酯(19)。结论:化合物1、3～5、7、15、17～19为首次从芫花条中分离得到。芫花条乙醇提物的石油醚萃取部位高剂量组(400 mg/kg)、氯仿萃取部位高剂量组(200 mg/kg)及咖啡酸正二十二烷酯对二甲苯致小鼠耳肿胀有显著的抑制作用。 |
| 关键词: 芫花 黄酮 木脂素类 抗炎活性 |
| DOI：10.3724/SP.J.1008.2008.01221 |
| 投稿时间：2008-03-25修订日期：2008-06-26 |
| 基金项目:上海市科技发展基金(04DZ19842). |
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| Anti-inflammatory constituents from the stems of Daphne genkwa |
| HE Lei, SHI Qi-rong, LIU Run-hui, XU Xi-ke, SHEN Yun-heng, LI Hui-liang, ZHANG Wei-dong* |
| (Department of Natural Medicinal Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective:To study the anti-inflammation contents in the stems of Daphne genkwa.Methods: The anti-inflammation contents were obtained from Daphne genkwa by bio-assay guide isolating method. The pharmacological model of dimethylbenzene-induced ear swelling was used for pharmacological study. The fractions of Ligroine and chloroform part of the the EtOH extraction were isolated and purified by column chromatography on silica gel and Sephadex LH-20 and recrystallization. Their structures were studied by using UV，IR,1H-NMR，13C-NMR， and MS, techniques.Results: Nineteen compounds were isolated from the stems of Daphne genkwa and were identified as 2,3-dihydroxypropyl hexadecoate(1), β-sitosterol(2), dueicosanyl caffeate(3), docosyl caffeate(4), octadecyl caffeate(5), daucosterol(6), genkwanin(7), luteolin(8), （+）lariciresinol(9), apigenin(10), kaempferol(11), daphnodorin B(12), genistein(13), dihydrokaempferol(14), p-hydroxybenzonic acid(15), quercetin(16), syringin(17), syringaldehyde(18), ethyl 4-hydroxybenzoate(19).Conclusion: Compounds 1, 3-5, 7, 15, 17-19 have been isolated from the stems of Daphne genkwa for the first time. The high dosage of petroleum ether extract, chloroform extract and compound 4 have significant anti-inflammation activity in mice with ear swelling. |
| Key words: Daphne genkwa flavone lignans anti-inflammatory activity |
