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| 胰腺导管内乳头状黏液性肿瘤组织DNMT1和HDAC1的表达及意义 |
| 王伟1,满晓华1,高军1,高丽2,龚燕芳1,李兆申1* |
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| (1.第二军医大学长海医院消化内科,上海 200433;2.第二军医大学长海医院病理科,上海 2004333) |
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| 摘要: |
| 目的:观察胰腺导管内乳头状黏液性肿瘤(IPMNs)组织DNA甲基化转移酶1(DNMT1)和组蛋白去乙酰化酶(HDAC1)的表达,探讨其可能的临床意义。方法:免疫组织化学SP法检测DNMT1、HDAC1蛋白在48例IPMNs和54例胰腺导管腺癌(PDAC)组织中的表达并进行统计学分析。结果:DNMT和HDAC1阳性表达率在由正常胰腺导管→IPMA,IPMB→IPMC→PDAC的逐级进展过程中均逐渐升高,二者在IPMNs不同亚型中均有阳性表达。结论:DNMT1和HDAC1高表达是胰腺癌的早期事件,二者的表达水平能够反映IPMNs的恶性进展,但不能作为IPMNs的组织分型标志。 |
| 关键词: 胰腺导管内乳头状黏液性肿瘤 DNA甲基化转移酶 组蛋白去乙酰化酶 免疫组织化学 |
| DOI：10.3724/SP.J.1008.2008.01303 |
| 投稿时间：2008-03-27修订日期：2008-07-22 |
| 基金项目: |
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| Expression of DNA methyltransferase 1 and histone deacetylase 1 proteins in intraductal papillary mucinous neoplasms |
| WANG Wei1,MAN Xiao-hua1,GAO Jun1,GAO Li2,GONG Yan-fang1,LI Zhao-shen1* |
| (1.Department of Gastroenterology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China; 2.Department of Pathology,Changhai Hospital,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective:To investigate the expression of DNA methylation transferase 1 (DNMT1) and histone deacetylase 1 (HDAC1) proteins in intraductal papillary mucinous neoplasms (IMPNs) and its possible clinical significance.Methods:The presence of DNMT1 and HDAC1 proteins in the 48 IPMNs and 54 pancreatic ductal adenocarcinomas (PDACs) were examined using SP immunohistochemical method and were statistically analyzed.Results:The expression levels of both DNMT1 and HDAC1 increased in a malignancy-dependent manner:normal pancreatic ductal →IPMA,IPMB → IPMC → PDAC.And both of them were positive in IPMNs of different subtypes.Conclusion:Higher expression of DNMT1 and HDAC1 is an early event of pancreatic malignancy.Expression of them can reflect the malignancy transformation of IPMNs,but can not be used for histological typing of IPMNs. |
| Key words: intraductal papillary mucinous neoplasms DNA methylation transferase histone deacetylase immunohistochemistry |
