| 摘要: |
| 目的:探讨叔丁醇外用滴眼液对SD大鼠硒性白内障模型(模拟老年性白内障)的治疗作用。 方法:建立SD大鼠硒性白内障模型,并将实验大鼠右眼作为药物干预组,左眼作为对照组；用自行配制的叔丁醇外用滴眼液(浓度分别为25、50、75 mmol/L)按时滴眼(1滴/次,3~5次/d)；每日进行肉眼观察,并于用药后第5日及第10日用裂隙灯显微镜观察大鼠双眼晶状体核性病变,每日用游标卡尺(最小刻度值0.02 mm)测量大鼠双眼晶状体核性斑块最大直径的改变。另选未建模的SD大鼠,用100 mmol/L的叔丁醇滴眼液滴眼(1滴/次,3~5次/d),观察药物有无毒性及其他不良反应。 结果:硒性白内障大鼠用药眼晶状体核性混浊斑块直径小于对照眼(P<0.01)；用药眼核性混浊斑块混浊程度低于对照眼；叔丁醇滴眼液毒性低,刺激性小。 结论:叔丁醇滴眼液对SD大鼠硒性白内障有一定的治疗作用。 |
| 关键词: 叔丁醇 烷基化作用 亚硒酸钠 白内障 |
| DOI：10.3724/SP.J.1008.2008.01056 |
| 投稿时间：2008-04-13修订日期：2008-05-28 |
| 基金项目:军队“十一五”资助课题(06MB213) |
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| Therapeutic effect of tertiary butyl alcohol eyedrops on sodium selenite-induced cataract in rats |
| LI Jing1, XUE Lian2, ZHANG Jun3, HUANG Cai-guo1, LI Wen-jie3* |
| (1.Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China2.Department of Clinical Laboratory, General Hospital of PLA, PLA Jinan Military Area Command,Jinan 250031 3.Central Laboratory, Changhai Hospital, Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective:To study the therapeutic effect of tertiary butyl alcohol (TBA) eyedrops on selenite-induced rat cataract (simulating senile cataract) .Methods: Rat cataract model was induced by sodium selenite. The right eyes of the rats were treated with 25, 50, or 75 mmol/L TBA eyedrops (1 drop/time, 3-5 times/day); the left eyes were taken as control. The eyes were observed everyday and were examined using slit lamp at the fifth and tenth day after treatment for nuclear plaques of the lens. The maximal diameter changes of the cataract plaques were detected using vernier cursor (with the minimum division value being 0.02 mm). Meanwhile, the right eyes of normal SD rats were stimulated with 100 mmol/L TBA eyedrops (1 drop/time, 3-5times/day) to investigate the toxic effect of the eyedrops and other adverse reactions.Results: The diameter and degree of cataract plaques of the nuclear cataract in the right eyes were less than those in the left eyes, and the opacification degree in the right eyes was slighter than that in the left eyes. The TBA eyedrops had less toxicity and stimulation to the eye.Conclusion: TBA eyedrops have obvious therapeutic effect on selenite cataract. |
| Key words: tertiary butyl alcohol alkylation sodium selenite cataract |
