| 摘要: |
| 目的：探讨抑制肺鳞癌细胞中Wnt5a表达对细胞增殖、迁移和侵袭等生物学特性的影响。方法：构建表达针对Wnt5a的siRNA的重组质粒pH1-siRNA Wnt5a-,转染肺鳞癌细胞SK-MES-1后筛选建立稳定表达细胞株。通过MTT、细胞周期以及Transwell检测,观察抑制Wnt5a表达对癌细胞增殖、迁移和侵袭能力的变化。结果：Western印迹测定表明肺鳞癌细胞SK-MES-1Wnt5a-低表达Wnt5a蛋白 (13.6%)。转基因细胞增殖指数略低于对照细胞株\[(28.3±3.8)% vs (30.5±5.2)%\]。转基因细胞的相对迁移率和侵袭率分别为对照组的 (47.3±9.2)%和 (39.7±11.7)%。结论：Wnt5a低表达显著抑制肺鳞癌细胞SK-MES-1的迁移和侵袭能力,Wnt5a可能是肺鳞癌基因治疗的潜在靶标。 |
| 关键词: Wnt5a RNA干扰 肺肿瘤 鳞状细胞肿瘤 细胞迁移 肿瘤侵袭 |
| DOI：10.3724/SP.J.1008.2008.00626 |
| 投稿时间：2008-04-15修订日期：2008-05-08 |
| 基金项目:上海市科委重点项目(071409011). |
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| Silence of Wnt5a gene inhibits migration and invasion of lung squamous carcinoma cells |
| WANG Wei-xing,MAO Yan-jun,YANG Ji-jin* |
| (Department of Radiology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective：To study the influence of Wnt5a gene silence on the proliferation,migration and invasion of lung squamous carcinoma cells.Methods: A recombinant plasmid pH1-siRNAWnt5a- was constructed and used to deliver small interference RNA (siRNA) targeting Wnt5a in SK-MES-1 cells; the transfected cells were screened to establish a stable transgenic cell line.MTT,cell cycle and Transwell assays were employed to evaluate the effect of Wnt5a gene silence on the proliferation,migration and invasion of lung squamous carcinoma cells.Results: Western blotting assay revealed that Wnt5a was lowly expressed in SK-MES-1Wnt5a-(13.6%).The proliferation index (PI) of transgenic cell line was slightly lower than that of the control cell line (\[28.3±3.8\]% vs \[30.5±5.2\]%).The migration and invasion capabilities of SK-MES-1Wnt5a- cells were (47.3±9.2)% and (39.7±11.7)% of the control cells,respectively.Conclusion: Low Wnt5a expression can significantly inhibit the migration and invasion capabilities of SK-MES-1 cells,indicating that Wnt5a might be a potential target for gene therapy of lung squamous carcinoma. |
| Key words: Wnt5a RNA interference lung neoplasms squamous cell neoplasms cell migration neoplasm invasiveness |
