| 摘要: |
| 目的:研究趋化因子受体CXCR4在肝细胞癌组织、肝癌MHCC97细胞、人脐带血管内皮细胞(HUVEC)中的表达，同时检测肝癌患者腹水中CXCR4的配体CXCL12的含量，为进一步探讨CXCR4在肝癌细胞转移中的作用奠定基础。方法:利用半定量RT-PCR和蛋白印迹方法检测MHCC97细胞、HUVEC细胞、21例不同时期的肝细胞癌组织及17例正常肝组织中CXCR4的表达水平，同时ELISA检测18例肝癌患者腹水中CXCL12的含量。结果:在肝细胞癌组织、肝癌细胞株及血管内皮细胞中，CXCR4在mRNA及蛋白水平的相对表达量分别为2.21±1.09、2.14±1.15、1.72±1.20及1.51±0.12、1.76±0.25、1.89±0.24，正常肝脏组织在mRNA及蛋白水平均未检测到CXCR4的表达。肝细胞癌癌性腹水定量检查结果显示，CXCL12含量为783~8 364 pg/ml(中位数为6 871 pg/ml)。结论:肝细胞癌组织和细胞中存在着CXCR4的高表达，但与肝癌临床分期无显著性相关，同时肝癌患者腹水中存在CXCL12的含量升高，这提示CXCR4可能在肝癌的转移中发挥重要的作用。 |
| 关键词: 肿肿瘤 肝细胞癌 MHCC97细胞 HUVE细胞 CXCR4受体 CXCL12 |
| DOI：10.3724/SP.J.1008.2008.00 |
| 投稿时间：2008-04-20修订日期：2008-06-25 |
| 基金项目: |
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| Expression of chemokine receptor CXCR4 in hepatocellular carcinoma |
| LIU Hui,ZHOU Wei-ping*,FU Si-yuan,WU Meng-chao |
| (The Third Department of Hepatic Surgery,Eastern Hepatobiliary Hospital,Second Military Medical University,Shanghai 200438,China) |
| Abstract: |
| Objective:To investigate the expression of chemokine receptor CXCR4 in hepatocellular carcinoma tissues,hepatocellular carcinoma cell line-MHCC97,human umbilical vein endothelial cells (HUVECs) and the ascites level of CXCL12,ligand of CXCR4,so as to lay a foundation for studying the role of CXCR4 in the metastasis of hepatocellular carcinoma.Methods: The expression of CXCR4 mRNA and protein was examined by RT-PCR and Western blotting in 21 specimens of hepatocellular carcinoma tissues,MHCC97 cells,HUVECs,and 17 specimens of normal hepatic tissues.Meanwhile,the levels of CXCL12 in ascitic fluids were assayed by ELISA in 18 hepatic cancer patients.Results: The relative expression values of CXCR4 mRNA in hepatocellular carcinoma tissues,MHCC97 cells,and HVECs were 2.21±1.09,2.14±1.15 and 1.72±1.20, respectively; and those of CXCR4 protein were 1.51±0.12,1.76±0.25, and 1.89±0.24,respectively; and those of CXCR4 protein were 1.51±0.12,1.76±0.25,and 1.89±0.24,respectively.CXCR4 mRNA and protein were not detected in normal hepatic tissues.ELISA results showed that the 18 hepatocellular carcinoma samples had a CXCL12 concentration range of 783-8 364 pg/ml (median value 6 871 pg/ml ) in ascitic fluids.Conclusion: CXCR4 is highly expressed in the hepatocellular carcinoma tissues and cells,which is not associated with the clinical staging of the cancer.The elevated CXCL12 level in the ascitic fluid of cancer patients indicate that CXCR4 may play an important role in the metastasis of hepatocellular carcinoma. |
| Key words: liver neoplasms hepatocellular carcinoma MHCC97 cell HUVEC cell CXCR4 receptors CXCL12 |
