| 摘要: |
| 目的:研究蛇莓乙酸乙酯萃取物的化学成分。方法:以95% 乙醇回流提取,对提取液的乙酸乙酯萃取物采用正相硅胶、反相硅胶、Sephadex LH-20柱色谱和高效液相色谱进行分离纯化,并根据理化性质和波谱数据鉴定化合物的化学结构。结果:分离得到13个化合物,分别鉴定为蔷薇酸(1),arjunic acid(2),对羟基桂皮酸(3),芹菜素(4),山柰酚(5),2α-羟基乌苏酸(6),2α-羟基齐墩果酸(7),委陵菜酸(8),刺梨苷(9),翻白叶苷A(10),野蔷薇苷(11),紫云英苷(12) 和异槲皮苷(13)。结论:化合物2~5、10、12均为首次从蛇莓属中分离得到。 |
| 关键词: 蛇莓 分离与纯化 中药鉴定 芹菜素 翻白叶苷A |
| DOI：10.3724/SP.J.1008.2008.01366 |
| 投稿时间：2008-05-06修订日期：2008-07-24 |
| 基金项目: |
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| Study on chemical constituents of Duchesnea indica Andr.Focke |
| MIAO Qing1,2,BAO Hai-yan1,2,PIAO Shu-juan1,LIN Hou-wen1*,QIU Feng2 |
| (Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;2.Department of Natural Medicinal Chemistry,School of Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016) |
| Abstract: |
| Objective:To investigate the chemical constituents in the EtOAc fraction of the EtOH extract of Duchesnea indica Andr.Focke.Methods: Extraction was done with 95% EtOH.EtOAc fraction of the EtOH extract was isolated and purified by column chromatography using silica gel,RP-C18,Sephadex LH-20 and HPLC.The structures of the compounds were identified by their physico-chemical properties and spectral analysis.Results: Thirteen compounds were obtained from the EtOAc fraction of Duchesnea indica Andr.Focke and they were identified as euscaphic acid(1),arjunic acid(2),p-hydroxy cinnamic acid(3),apigenin(4),kaempferol(5),2α-hydroxy ursolic acid(6),2α-hydroxy oleanolic acid(7),tomentic acid(8),kaii-ichigeside F1(9),potengriffioside A(10),rosamultin(11),kaempferol-3-O-β-D-glucoside(12),and isoquercitrin(13).Conclusion: The compounds 2-5,10,and 12 have been isolated from the genus of Duchesnea for the first time. |
| Key words: Duchesnea indica isolation and purification TCD identification apigenin potengriffioside A |
