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| MPTP对快速老化小鼠SAMP8学习记忆能力及黑质多巴胺神经元的影响 |
| 苑振云1,2,顾平2,3,籍文强1,崔冬生2,刘力2,王彦永2,3,耿媛2,王铭维2,3* |
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| (1.河北医科大学第一医院急诊科,石家庄050031;2.河北省脑老化与认知神经科学实验室,石家庄050031 ; 3.河北医科大学第一医院神经内科,石家庄050031) |
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| 摘要: |
| 目的:探讨1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)对SAMP8小鼠空间学习和记忆能力及黑质多巴胺神经元的影响。方法:采用雄性3个月龄 SAMP8小鼠皮下注射MPTP(36 mg/kg),连续5 d。对照组皮下注射生理盐水（NS，36 ml/kg)。用Morris水迷宫测试小鼠的寻找平台潜伏期及搜索策略。免疫组织化学观察黑质酪氨酸羟化酶(TH)阳性多巴胺能神经元变化。结果:MPTP小鼠黑质致密部TH免疫反应阳性神经元数目明显少于对照组(P<0.01)。 Morris水迷宫实验表明MPTP小鼠寻找平台潜伏期较对照组明显延长(P<0.01),在目标象限(第Ⅰ象限)游泳时间比对照组明显降低(P<0.01),在对侧象限(第Ⅳ象限) 游泳时间比对照组明显升高(P<0.05),搜寻策略较对照组变差。 结论:MPTP可造成SAMP8小鼠黑质多巴胺神经元损伤,并出现空间学习记忆能力下降。 |
| 关键词: MPTP SAMP8 多巴胺能神经元 学习 记忆 |
| DOI：10.3724/SP.J.1008.2008.01337 |
| 投稿时间：2008-05-15修订日期：2008-10-13 |
| 基金项目: |
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| Effects of MPTP on spatial learning ability/memory and dopaminergic neurons in Nigra of senescence accelerated-prone 8 mice |
| YUAN Zhen-yun1,2,GU Ping2,3,JI Wen-qiang1,CUI Dong-sheng2 ,LIU Li2,WANG Yan-yong2,3,GENG Yuan2,WANG Ming-wei2,3* |
| (1.Department of Emergency,First Hospital of Hebei Medical University,Shijiazhuang 050031,China;2.Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province,Shijiazhuang 050031;3.Department of Neurology,First Hospital of Hebei Medical University,Shijiazhuang 050031) |
| Abstract: |
| Objective:To observe the effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) on the spatial learning ability/memory and dopaminergic neurons in the Nigra of senescence accelerated-prone 8 (SAMP8) mice.Methods: Three-month old male SAMP8 mice were injected with MPTP (36 mg/kg,s.c.) for 5 days,and animals in the control group were injected with NS (36 ml/kg,s.c.) in the same manner.Morris water maze was used to examine the searching strategy,seeking-platform latency,and the swimming time in the aimed quadrant.Immunohistochernistry was used to observe the changes of TH-ir positive neurons in substantia nigra.Results: The number of TH-ir neurons in substantia nigra pars compacta was significantly reduced in MPTP group compared with the control group(P<0.01).Morris water maze showed that the searching strategy of animals in MPTP group was worse than in the control group,with the seeking-platform latency of MPTP mice significantly prolonged (P<0.01),the time spent in the aimed quadrant significantly decreased (P<0.01) and time in the opposite quadrant significantly prolonged (P<0.05).Conclusion: MPTP can cause damage to the dopaminergic neurons in the substantia nigra of SAMP8 mice,which is subsequently followed by deficit in the spatial learning and memory in the animals. |
| Key words: MPTP SAMP8 dopaminergic neuron learning memory |
