| 摘要: |
| 目的:观察骨癌痛模型大鼠脊髓背根神经节中P2X3受体及其mRNA的表达变化，初步探讨其可能的意义。方法:60只雌性Wistar大鼠随机分为3组（每组n=20）：空白对照（C）组；骨癌痛模型（CP）组，左胫骨内接种含2×105个Walker-256大鼠乳腺癌细胞的悬液10 μl；假手术（S）组，左侧胫骨骨髓腔内注入等体积的生理盐水。在接种后第4、7、10、14、17、21天进行疼痛行为学测试，在第14、21天各组分别选10只大鼠，取背根神经节，5只行免疫组化染色，另5只抽提RNA行实时PCR，检测背根神经节中P2X3受体及其mRNA的表达。结果:CP组大鼠在胫骨内接种肿瘤后第10天开始出现痛觉过敏，第14~21天最为明显。CP组接种肿瘤后第14、21天患侧背根神经节神经元中P2X3受体免疫阳性细胞率明显增高（P<0.05），mRNA表达水平显著增高（P<0.05）。结论:骨癌痛模型大鼠存在痛觉敏化,可能与P2X3受体表达增高有关。 |
| 关键词: 骨癌痛 背根神经节 嘌呤能受体 大鼠 |
| DOI：10.3724/SP.J.1008.2009.0244 |
| 投稿时间：2008-06-27修订日期：2008-12-21 |
| 基金项目: |
|
| Changes of P2X3 receptor expression in dorsal root ganglia in a rat model of bone cancer pain |
| WU Jing-xiang1, XU Mei-ying2, MIAO Xue-rong1, LI Xiao-qing1, YU Wei-feng1* |
| (1.Department of Anesthesiology, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, China;2.Department of Anesthesiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030) |
| Abstract: |
| Objective:To observe the immunoreactive changes of P2X3 receptor and examine P2X3 receptor mRNA level in the dorsal root ganglia (DRG) in a rat model of bone cancer pain. Methods: Sixty female Wistar rats, weighing 150-200 g, were randomly allocated to three groups (n=20 each): group CP (cancer pain) received intra-tibial injection of Syngeneic Walker 256 mammary gland carcinoma cells (2×105 ), group S received intra-tibial injection of equal volume 0.9% sodium chloride, and group C received blank control. Mechanical allodynia and thermal hyperalgesia tests were taken on 4, 7, 10, 14, 17, and 21 days after inoculation. On day 14 and 21, ten rats of each group were chosen for either immunohistochemistry or real-time RT-PCR to examine the changes of P2X3 receptor and mRNA. Results: The rats in group CP began to display hyperalgesia on day 10 and the pain became most obvious between day 14 and 21. Immunohistochemistry showed that the proportion of DRG neurons positive of P2X3 receptor in group CP was significantly increased on 14 and 21 d(P<0.05), and RT-PCR showed that the expression of P2X3 receptor mRNA was also significantly increased. Conclusion: These results suggest that our rat model of bone cancer pain has hyperalgesia, which might be related to the increase of P2X3 receptor expression. |
| Key words: bone cancer pain dorsal root ganglia purine receptors hyperalgesia |
