| 摘要: |
| 目的:探讨骨肉瘤乳腺癌特异基因1（breast carcinoma specific gene 1,BCSG1）、B细胞淋巴瘤/白血病-2（B cell lymphoma/leukemia-2，Bcl-2）、Bcl-2相关转录因子（Bcl-2-associated transcription factor，BTF）和雌激素受体（estrogen receptor，ER）基因表达水平及意义。方法:用实时荧光定量RT-PCR技术检测16例低(未)分化骨肉瘤标本及成骨细胞系hFOB 1.19细胞株BCSG1、BTF、Bcl-2、ERα、ERβ mRNA的表达，分析各指标间的相关性。结果:骨肉瘤组织中BCSG1、Bcl-2、ERα、ERβ的表达明显高于成骨细胞系hFOB 1.19细胞株（P<0.01），BTF表达两组无统计学差异；骨肉瘤组织ERα/ERβ显著高于成骨细胞系hFOB 1.19细胞株（P<0.01）；肿瘤瘤体大小、患者性别对BCSG1、Bcl-2、BTF、ERα、ERβ的表达未见显著影响；骨肉瘤组织中BCSG1与ERα（r=0.696,P<0.01）、ERβ与Bcl-2（r=0.875,P<0.01）、ERα/ERβ与Bcl-2基因表达水平（r=-0.649,P<0.01）分别存在线性关系。结论:骨肉瘤组织中可能存在BCSG1增强ERα基因转录激活的作用，ERβ可能影响Bcl-2的表达。 |
| 关键词: 乳腺癌特异基因 Bcl-2 雌激素受体 转录因子 骨肉瘤 |
| DOI：10.3724/SP.J.1008.2009.0416 |
| 投稿时间：2008-07-03修订日期：2009-03-02 |
| 基金项目:上海市基础研究重点项目（04JC14028）. |
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| mRNA expression of BCSG1, BTF, Bcl-2, ERα and ERβ in osteosarcoma and its significance |
| LEI Ming1, MIAO Ming-yong2, XU Wei-dong1* |
| (1.Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;2.Department of Biochemistry & Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433) |
| Abstract: |
| Objective:To observe the mRNA expression of BCSG1（breast carcinoma specific gene,BCSG1）, BTF（Bcl-2-associated transcription factor，BTF）, Bcl-2（B cell lymphoma/leukemia-2，Bcl-2） and two subtypes of estrogen receptors in osteosarcoma and their implication. Methods: The expression of BCSG1, ERα, BTF, ERβ, and Bcl-2 mRNA were examined in 16 poorly differentiated osteosarcoma samples and a cell line hFOB 1.19 by quantitative RT-PCR. The relationship between their expression was analyzed. Results: The mRNA expression of BCSG1, Bcl-2 and two subtypes of estrogen receptors in the osteosarcoma tissues was significantly higher than that in the osteoblast cell line of hFOB 1.19; the expression of BTF was not significantly different. ERα/ERβ in the osteosarcoma tissues was significantly higher than that in the hFOB 1.19 cells (P<0.01). BCSG1, ERα, ERβ, Bcl-2 and BTF expression was not associated with the tumor size or patients gender. Correlation analysis showed there was a linear relationship between expression of BCSG1 and ERα，ERβ and Bcl-2, and ERα/ERβ ratio and Bcl-2. Conclusion: BCSG1 may enhance the activation of estrogen receptor-α gene transcription, and ERβ may influence the expression of Bcl-2 in osteosarcoma. |
| Key words: breast carcinoma specific gene Bcl-2 estrogen receptor transcription factors osteosarcoma |
