| 摘要: |
| 目的:探讨性别差异对脓毒症大鼠血小板活化的影响。方法:40只健康SD大鼠(雌雄各半)随机分为4组(n=10)：雄性假手术组、雌性假手术组、雌性脓毒症组、雄性脓毒症组。后两组盲肠结扎穿孔(CLP)法制作脓毒症动物模型，假手术组不行盲肠结扎穿孔。术后24 h留取大鼠血液标本，行血小板计数检测，采用ELISA方法检测β血小板球蛋白(β-TG)表达情况，采用放射免疫测定法检测大鼠血清雌二醇(E2)含量。结果:脓毒症雌、雄性大鼠血小板计数均下降，但雌性大鼠明显高于雄性(P<0.01)。脓毒症雌、雄性大鼠血清β-TG表达均升高，但是雌性大鼠表达量明显低于雄性大鼠(P<0.01)。雌、雄性脓毒症大鼠全血血小板计数与相应性别大鼠 E2水平显著正相关，血清β-TG水平与相应性别大鼠 E2水平显著负相关(P<0.05)。结论:脓毒症的血小板活化存在性别差异，内源性雌激素可能介导了对雌性脓毒症大鼠血小板的保护作用。 |
| 关键词: 血小板 脓毒症 性别因素 雌激素类 |
| DOI：10.3724/SP.J.1008.2009.0249 |
| 投稿时间：2008-08-06修订日期：2008-09-21 |
| 基金项目: |
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| Gender difference in activation of platelets in septic rats |
| HU Jin-yu, LI Jin-bao, DENG Xiao-ming* |
| (Department of Anaesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective:To investigate the influence of gender difference on the activation of platelets in septic rats.Methods: Totally 20 female and 20 male SD rats were randomly divided into four groups: male sham-operated group, female sham-operated group, female septic group, and male septic group, with each group containing 10 rats．Sepsis model in the last two groups was produced by cecal ligation and puncture (CLP); rats in the sham-operated group received no CLP．The rats were sacrificed 24 hours after surgery; the blood samples were collected for blood routine test; and the serum β-TG levels were detected by ELISA assay. Serum estrogen (E2) levels were detected by radioimmunoassay.Results: The blood platelet counts were decreased in both septic rats of both genders; the count in female rats was significantly higher than in the male rats (P<0.01). The serum β-TG levels were increased in septic rats of both genders; the level in the female rats was significantly lower than that in the male rats (P<0.01). The blood platelets count in female or male septic rats was significantly positive correlation with the level of serum estrogen and the β-TG expression in female or male septic rats was significantly negative correlation with the level of serum estrogen(P<0.05).Conclusion: There is a gender difference in the activation of platelets in septic rats, and the endogenous estrogen may have protective effect on the platelets of female septic rats. |
| Key words: blood platelets sepsis gender factors estrogens |
