| 摘要: |
| 目的:探讨寻常型天疱疮（PV）患者外周血T细胞受体（TCR）互补决定区（CDR3）基因谱型变化，了解克隆扩增的T细胞与自身免疫性疾病的相互关系。方法:采用免疫扫描谱型分析技术，分析6例正常人外周血单个核细胞TCR β链可变区(BV) CDR3谱型分布特征以及6例PV患者CDR3谱型变化情况。结果:6例正常人TCR BV CDR3谱型均符合正态分布，6例PV患者TCR BV CDR3谱型均出现多态性降低，BV亚家族单/寡克隆增生。结论:PV患者外周血TCR可变区β链CDR3谱型异常可能与PV发病有关。 |
| 关键词: 寻常型天疱疮 T淋巴细胞受体 互补决定区3 基因多态性 |
| DOI：10.3724/SP.J.1008.2009.0291 |
| 投稿时间：2008-09-11修订日期：2008-10-21 |
| 基金项目:国家自然科学基金（30771937）. |
|
| Spectratyping analysis of complementarity-determining region 3 of T-cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with pemphigus vulgaris |
| WANG Qian1, ZENG Kang1*, ZHOU Hao2, SUN Le-dong1, ZHOU Zai-gao1 |
| (1. Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2. Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515) |
| Abstract: |
| Objective:To analyze the changes of the complementarity-determining region 3(CDR3) of T-cell receptor (TCR) beta chain variable region (TCR BV) in peripheral blood mononuclear cells (PBMCs) of patients with pemphigus vulgaris (PV), so as to understand the association of proliferated T cells with autoimmune diseases.Methods: Immunoscope spectratyping technique was used to analyze the distribution of TCR β chain CDR3 in 6 normal blood donors and the dominant CDR3 in the PBMCs in 6 PV patients. Results: The spectratypes of TCR BV subfamily CDR3 region was in a Gaussian distribution manner in all the 6 normal blood donors. The 6 PV patients, however, displayed abnormal proliferation, and oligoclonal expansion of the T cells was observed in TCR BV families with different CDR3 sequences. Conclusion: The abnormal spectratypes of TCR BV subfamily CDR3 region in the peripheral blood of PV patients might be related to the pathogenesis of PV. |
| Key words: pemphigus vulgaris T cell receptor complementarity determining region 3 genetic polymorphism |
