| 摘要: |
| 目的:通过基因芯片技术筛查与宫颈癌发生密切相关的转录因子,并在蛋白水平进行验证；初步探讨其在宫颈癌发生中的临床病理意义。方法:对新鲜宫颈癌、癌旁组织及正常宫颈组织各2例进行人表达谱芯片（Illumine Human-6 gene chip, 含48 000个基因信息）检测，采用实时定量 RT-PCR方法验证芯片结果，筛选出调控差异基因主要转录因子。并进一步对44例宫颈癌、22例宫颈内上皮瘤变及17例正常宫颈石蜡组织进行免疫组化检测，验证基因芯片筛选的主要转录因子表达情况，分析转录因子和宫颈癌临床病理学关系。结果: 癌组织、癌旁组织与正常宫颈组织间比较共同上调基因67个，下调基因28个，RT-PCR验证了基因芯片结果。其中，NF-κB p65在癌旁组织和正常组织中低表达，在宫颈癌组织中高表达（P<0.05），NF-κB p65与宫颈癌组织分级呈正相关（P<0.05），与临床分期无关（P>0.05）；Foxq1在癌旁和正常组织中高表达，在宫颈癌组织中阴性表达（P<0.05），Foxq1表达与宫颈癌组织分级、临床分期均无关（P>0.05）。Foxq1与NF-κB p65表达无关（P>0.05）。结论:核因子NF-κB p65与Foxq1为宫颈癌发生相关转录因子，可能分别在宫颈癌的发生中起作用。 |
| 关键词: 宫颈肿瘤 NF-κB p65 Foxq1 基因芯片 转录因子 |
| DOI：10.3724/SP.J.1008.2009.0300 |
| 投稿时间：2008-09-18修订日期：2009-01-19 |
| 基金项目:浙江省湖州市自然科学基金(2005YZ14);浙江省医药卫生科学研究基金 (2005A096). |
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| Expression of Foxq1 and NF-κB p65 in cervical cancer and its clinical pathological significance |
| ZHANG Su,XING Jian-ming,QIAN Cheng,DENG Zai-xing,CHENG Hong-ling |
| (Maternity and Child Care Hospital of Huzhou, Huzhou 313000, China) |
| Abstract: |
| Objective:To screen for the transcription factor (TF) closely related to the development of cervical cancer tissue by microarrry gene chip and to testify the results at protein level, so as to assess clinico-pathologic significance of these TFs. Methods: The differential genes in 2 fresh cervical cancer specimens and their adjacent normal tissues and 2 normal cervical tissues were examined by Illumine Human-6 gene chip. The expression of the important TFs selected was verified by RT-PCR, and the major TFs regulating the differential genes were selected. The selected TFs were further investigated in 44 cervical cancer tissues, 22 cervical intraepithelial neoplasia(CIN), and 17 normal cervical tissues using immunohistochemical method. The relationship between the expression of major TFs in cervical cancer and the clinico-pathological characteristics was evaluated. Results: There were 67 upregulated genes and 28 downregulated genes in cervical cancer tissue and CIN when compared to normal tissue, these were testified by RT-PCR. Among these genes,the expression of NF-κB p65 in cervical cancer tissues was up-regulated compared with that in the CIN tissues and normal tissues (P<0.05). Expression of NF-κB p65 was positively correlated with the pathological grade of cervical cancer (P<0．05), and was not associated with FIGO stage (P>0．05). The expression of Foxq1 in cervical cancer was significantly lower than that in CIN and normal tissues (P<0．05), and the expression was not correlated with FIGO stage, pathological grade, or NF-κB p65 expression (P>0.05). Conclusion: NF-κB p65 and Foxq1 are associated with the development of cervical cancer; they may play different roles in the oncogenesis of cervical cancer. |
| Key words: cervix neoplasms NF-κB p65 Foxq1 microarray chip transcription factor |
