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乳腺癌组织中N-乙酰氨基葡萄糖转移酶Ⅴ的活性和临床意义
胡薇1,施俊义1,郑唯强2,倪灿荣2,陈颖2,于观贞3,盛湲1,李莉1,方国恩1*
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(1.第二军医大学长海医院普通外科,上海 200433;2.第二军医大学长海医院病理科,上海 200433;3.第二军医大学长征医院肿瘤科,上海 200003)

摘要:

目的:检测乳腺癌组织中N乙酰氨基葡萄糖转移酶Ⅴ（GnTⅤ）的活性并分析其临床意义。方法: 应用组织芯片技术构建12×10乳腺癌组织阵列块,通过凝集素印迹法检测以白细胞植物凝集素(PHAL)标记的GnTⅤ在57例乳腺癌和 12 例乳腺良性病变中的活性,并探讨其与临床病理学参数间的关系。结果: 乳腺癌组织中PHAL阳性89.47％(51/57),胞质阳性,乳腺良性病变组织中PHAL阳性8.33％(1/12),两者存在显著性差异(P<0.01)。乳腺癌中GnTⅤ活性在不同病理类型、不同淋巴结转移状况、不同病理分期患者中的表达无明显差异。结论: 乳腺病灶GnTⅤ高活性提示病变恶性可能大,可以GnTⅤ为靶点,治疗出现复发及转移三阴的乳腺癌患者。

关键词: 乳腺肿瘤；癌 N 乙酰氨基葡萄糖转移酶Ⅴ；组织芯片

DOI：10.3724/SP.J.1008.2009.0767

投稿时间：2008-09-20修订日期：2009-02-26

基金项目:

Activity of N acetylglucosaminyltransferase Ⅴ in breast cancer tissues and its clinical significance

HU Wei1,SHI Jun-yi1,ZHENG Wei-qiang2,NI Can-rong2,CHEN Ying2,YU Guan-zhen3,SHENG Yuan1,LI Li1,FANG Guo-en1*

(1.Department of General Surgery,Changhai Hospital,Second Military Medical University,Shanghai 200433,China; 2.Department of Pathology,Changhai Hospital,Second Military Medical University,Shanghai 200433;3.Department of Oncology,Changzheng Hospital,Second Military Medical University,Shanghai 200003)

Abstract:

Objective:To explore the activity of Nacetylglucosaminyltransferase Ⅴ（GnTⅤ） in breast cancer tissues and to discuss its clinical significance.Methods:The activities of GnTⅤwere examined in a tissue microarray cohort of 57 breast carcinomas and 12 benign breast lesions.Lectin blotting using Phaseolus vulgaris leukocytic phytohemagglutinin (PHAL) labeling was used to assay GnTⅤactivity.The relationship between clinicopathological parameters and GnTⅤactivity was investigated.Results:High activity of GnTⅤwas observed in 89.47% (51/57) breast cancer tissues,which was significantly higher than that in the benign breast lesions (8.33％\[1/12\],P<0.01).No difference in GnTⅤactivities was observed in breast cancer patients with different pathological types,different lymphatic statuses,and different stages.Conclusion:Higher GnTⅤactivity in breast cancer tissues indicates a higher risk of malignancy.Targeting GnTⅤmight be a future way for treatment of triplenegative breast cancer patients with recurrence and metastasis.

Key words: breast neoplasms carcinoma N-acetylglucosaminyltransferase Ⅴ tissue microarray