| 摘要: |
| 目的:采用转化生长因子(TGF-β1)联合肌成纤维细胞构建组织工程心脏瓣膜(TEHV),探讨TGF-β1能否改善TEHV的力学性能及其可能机制。方法:分离培养大鼠肌成纤维细胞,细胞鉴定后种植于去细胞瓣叶支架上。将瓣叶置于含10 ng/ml TGF-β1的培养液中,培养14 d构建的TEHV做为实验组,对照组除培养液中不添加TGF-β1外,其余同实验组。检测两组TEHV的赖氨酰氧化酶(LOX)和羟脯氨酸含量,LOX和Ⅰ型胶原(COLL-1) mRNA表达,以及力学性能。结果:实验组TEHV的LOX和羟脯氨酸含量均高于对照组(P<0.05),LOX和COLL-1 mRNA表达均高于对照组(P<0.05),最大负荷、最大应力均高于对照组(P<0.05),而两组的最大应变和弹性模量的差异无统计学意义(P>0.05)。结论:在体外构建TEHV过程中,通过TGF-β1对肌成纤维细胞的调控作用,可以在基因水平增强细胞COLL-1和LOX mRNA的表达,使得胶原蛋白合成和交联程度增加,从而使TEHV的力学强度增加,力学性能得到改善。 |
| 关键词: 转化生长因子β1 组织工程 心脏瓣膜 赖氨酰氧化酶 胶原 力学性能 |
| DOI：10.3724/SP.J.1008.2009.0124 |
| 投稿时间：2008-09-22修订日期：2008-12-05 |
| 基金项目:国家自然科学基金(30571839,30600608). |
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| Regulatory mechanism of transforming growth factor-beta 1 on mechanical properties of tissue engineered heart valve |
| HONG Hao,DONG Nian-guo*,SHI Jia-wei |
| (Department of Cardiovascular Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China) |
| Abstract: |
| Objective:To observe whether transforming growth factor-beta 1 (TGF-β1) can improve the mechanical properties of tissue engineered heart valve (TEHV) prepared by TGF-β1 and myofibroblasts.Methods: Myofibroblasts were isolated,cultured,identified,and seeded onto the decellularized aortic valve leaflet.In the experimental group,TEHV were cultured with DMEM containing 10 ng/ml TGF-β1 for 14 days.In the control group,TEHV were cultured with DMEM only for 14 days.The contents of lysyl oxidase (LOX) and hydroxyproline,the expression of LOX and COLL-1 mRNA,and the mechanical properties of the TEHVs were analyzed in each group.Results: The contents of LOX and hydroxyproline,expression of LOX and COLL-1 mRNA,Max-load,and Max-stress were significantly higher in the experimental group than those in the control group (all P<0.05).However,the experimental and control group showed comparable values of Max-strain and elastic modulus.Conclusion: TGF-β1 can increase expression of LOX and COLL-1 mRNA in the seeded cells during TEHV construction,increasing the production and cross-linking of collagen,and improve the mechanical properties of the TEHV. |
| Key words: transforming growth factor-beta 1 tissue engineering heart valve lysyl oxidase collagen mechanical properties |
