| 摘要: |
| 目的:探讨磁性聚乳酸羟基乙酸氧化苦参碱纳米粒(M-PLGA-OM-NP)对小鼠肝纤维化的防治作用。方法:运用复乳法制备M-PLGA-OM-NP；透射电镜观察纳米粒形态。采用二甲基亚硝胺(DMN)诱导小鼠肝纤维化模型,观察M-PLGA-OM-NP干预后肝脏生化指标及病理组织改变。 结果:M-PLGA-OM-NP外观呈规则球形,平均粒径为146.5 nm,载药量为7.61%,包封率为44.8%。M-PLGA-OM-NP组(外加磁场组和非磁场组)的丙氨酸转氨酶(ALT)水平较模型组下降；光镜下肝脏的纤维化程度较模型组明显减轻,较单纯氧化苦参碱(OM)用药组也明显改善；肝星状细胞活化的标志物α-SMA的表达较模型组及单纯OM用药组明显减弱。结论:M-PLGA-OM-NP在外加磁场的作用下,可增强OM的对DMN诱导的肝纤维化的防治作用。 |
| 关键词: 肝纤维化 氧化苦参碱 聚乳酸羟基乙酸 磁性纳米粒 |
| DOI：10.3724/SP.J.1008.2009.0500 |
| 投稿时间：2008-10-14修订日期：2009-02-24 |
| 基金项目:上海市卫生局科技发展基金(054123). |
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| Preparation of magnetic poly D,L-lactide-co-glycolide oxymatrine nanoparticles and their preventive effect against liver fibrosis in mice |
| ZHENG Zhi-wu1,CHEN Yue-xiang1,CHEN Wei-zhong1*,SONG Chun-yan1,QIAN Hui1,GAO Jing2 |
| (1.Department of Gastroenterology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;2.Department of Pharmaceutics,School of Pharmacy,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective:To prepare magnetic poly D,L-lactide-co-glycolide oxymatrine nanoparticle(M-PLGA-OM-NP) and investigate its preventive effects against experimental liver fibrosis.Methods: The M-PLGA-OM-NP was prepared by multiple emulsion process and observed by transmission electron microscope(TEM).Dimethylnitrosamine (DMN)-induced liver fibrosis was established with mice.After intervention with M-PLGA-OM-NP,the expression of α-smooth muscle actin (a-SMA) in the livers of mice was detected by immunohistochemical assay and the score of liver fibrosis was determined by H-E staining and Van Gieson (VG) staining.Results: The prepared M-PLGA-OM-NP was in a regular sphere shape,with a mean diameter of 146.5 nm; the drug loading was 7.61% and the encapsulation ratio was 44.8%.The serum Alt level in the M-PLGA-OM-NP group was decreased compared with that in the model group.Light microscopy revealed that the fibrosis in the M-PLGA-OM-NP group was greatly improved compared with the model group and pure oxymatrine group.The expression of a-SMA,the marker of hepatic stellate cell activation,was obviously decreased in the M-PLGA-OM-NP group compared with that in the other groups.Conclusion: The prepared M-PLGA-OM-NP under magnetic field can reinforce the preventive effect of oxymatrine against DMN-induced experimental liver fibrosis in mice. |
| Key words: liver fibrosis oxymatrine poly D,L-lactic-co-glycolide acid magnetic nanoparticles |
