| 摘要: |
| 目的:从噬菌体随机七肽库中筛选能与PreS1抗原特异性结合的多肽。方法:利用噬菌体展示技术,以PreS1抗原为靶分子,从随机七肽库中进行生物亲和筛选,ELISA鉴定阳性克隆。结果:对肽库进行3 轮筛选后,通过ELISA和竞争抑制ELISA,获得了特异性的结合肽,测序结果显示噬菌体上的短肽有共同序列。结论:利用噬菌体展示技术成功筛选到了PreS1抗原的结合肽,为乙肝的治疗和诊断探索新的途径。 |
| 关键词: PreS1抗原 噬菌体展示肽库 结合肽 乙型肝炎 |
| DOI：10.3724/SP.J.1008.2009.0783 |
| 投稿时间：2008-11-10修订日期：2009-05-11 |
| 基金项目: |
|
| Selection of peptides bind to PreS1 antigen from a phage-displayed peptide library |
| CHEN Qing-qing,ZHENG Hui-min,GUO Bao-yu* |
| (Department of Biochemical Pharmacology,School of Pharmacy,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To screen for peptides that specifically bind to PreS1 antigen from a phage-displayed peptide library.Methods: The PreS1 antigen was used as the target molecule to screen the binding peptide from the Ph.D.-7 peptide library with phage display technique,and the positive clones were identified by ELISA.Results: After three rounds of biopanning,the binding peptides were screened from the peptide library by ELISA and competitive inhibiting ELISA.Sequencing result showed that the binding peptides had high affinity and specificity.Conclusion: A peptide binding PreS1 antigen has been successfully obtained by screening the phage display library,which paves a way for the diagnosis and treatment of hepatitis B infection. |
| Key words: PreS1 antigen phage display peptide library binding peptide hepatitis B |
