| 摘要: |
| 目的:观察舒芬太尼对大鼠腹主动脉平滑肌细胞(AASMCs)钙激活钾电流(IKCa)的影响，探讨其扩张血管的作用机制。方法:用酶消化法急性分离AASMCs，采用全细胞膜片钳技术记录IKCa，观察不同浓度(1×10-8、3×10-8、1×10-7mol/L)舒芬太尼对该电流的影响。结果:与不加药对照组相比舒芬太尼能显著增大IKCa幅度(P<0.05或P<0.01)，这一作用具有可逆性，并具有浓度依赖性。结论:舒芬太尼能增强AASMCs钙激活钾通道的活动，这一作用可能与临床上观察到的舒芬太尼舒血管效应有关。 |
| 关键词: 舒芬太尼 腹主动脉 平滑肌细胞 钙激活钾通道 |
| DOI：10.3724/SP.J.1008.2009.01062 |
| 投稿时间：2008-11-24修订日期：2009-02-25 |
| 基金项目:国家自然科学基金(30772092). |
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| Effect of sufentanil on calcium-activated potassium currents in rat abdominal aortic smooth muscle cells |
| LIU Zhu-qing1,RONG Wei-fang2*,SHI Xue-yin1* |
| (1.Department of Anesthesiology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;2.Department of Physiology,School of Medicine,Shanghai Jiaotong University，Shanghai 200025) |
| Abstract: |
| Objective:To investigate the influence of sufentanil on calcium-activated K+ channels (IKCa) in rat abdominal aortic smooth muscle cells,and to investigate its role in dilation of blood vessels.Methods: Rat abdominal aortic smooth muscle cells (AASMCs) were freshly obtained by enzymatic digestion.Whole-cell voltage-clamp technique was used to assess the effect of sufentanil (1×10-8,3×10-8,1×10-7mol/L ) on IKCa.Results: Sufentanil significantly increased the amplitude of IKCa compared with the control group (P<0.05 or P<0.01).The effect of sufentanil was reversible and in a concentration-dependent manner.Conclusion: Sufentanil can promote the activation of KCa channel in rat AASMCs,which might be related to the vasodilatory effect of sufentanil observed in clinical practice. |
| Key words: sufentanil abdominal aorta smooth muscle myocytes calcium-activated potassium channels |
