| 摘要: |
| 目的:观察依达拉奉对脊神经结扎(SNL)大鼠痛敏的影响及其作用机制。方法:将SD雄性大鼠随机分为假手术(Sham)组、SNL模型组和依达拉奉(Eda)组,观察脊神经结扎大鼠术前及术后7 d机械痛反应阈值(PWMT)的变化；在术后相应时间点取各组大鼠手术侧L5和L4及对侧L5背根神经节(DRG)和脊髓,观察pJNK在DRG和脊髓中的表达分布及依达拉奉对pJNK表达的影响。结果:依达拉奉可以减轻脊神经结扎引起的痛敏现象；SNL组术后24 h手术侧L5DRG中pJNK阳性神经元的表达增加,术后3 d免疫双荧光显示脊髓中 pJNK在星型胶质细胞中存在高表达现象；依达拉奉可以降低相应时间点DRG和脊髓中pJNK表达的含量。结论:依达拉奉能抑制脊神经结扎引起的痛敏现象,其机制可能是通过降低脊髓和DRG中pJNK的含量而产生镇痛作用的。 |
| 关键词: 依达拉奉 自由基清除剂；脊神经结扎；神经痛；pJNK |
| DOI：10.3724/SP.J.1008.2009.0898 |
| 投稿时间：2008-12-02修订日期：2009-02-16 |
| 基金项目: |
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| Edaravone inhibits pain sensitivity through decreasing pJNK expression in dorsal root ganglia and spinal cord in rats with spinal nerve ligated |
| LIAO Xing-zhi1,LI Jie1,MAO Yan-fei2,YANG Xiao-ning1,XIONG Yuan-chang1* |
| (1.Department of Anesthesiology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;2.Department of SICU,Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200092) |
| Abstract: |
| Objective:To investigate the effect of edaravone on the pain sensitivity in rats with spinal nerve ligated and to probe into the related mechanism.Methods:Male adult SD rats were randomly divided into 3 groups:a sham(Sham) group,a spinal nerve ligation (SNL) group and edaravone(Eda) group.The paw withdrawal mechanical threshold(PWMT)was measured before and after ligation (once daily for 7 days).Rats were sacrificed at specified time points and the left(operation side) L4 and L5 dorsal root ganglia(DRG) and the right(control side) L5 DRG were obtained and immunostained to observe the changes of pJNK in DRG neurons and spinal cords,so as to observe the effect of edaravone on pJNK.Results:Edaravone can reduce the mechanical hyperalgesia induced by spinal nerve ligation.Immunostaining showed that the SNL group had an increased pJNK in the ipsilateral DRG neurons (L5) 24 hours after ligation; double immunofluorescence indicated that the expression of pJNK in the ipsilateral spinal astrocytes was increased 3 days after ligation.Edaravone can reduce pJNK expression in DRG neurons and spinal cords at corresponding time points.Conclusion:Edaravone can relieve the neuropathic pain induced by spinal nerve ligation,and the mechanism might be related to the inhibition of pJNK expression in DRG neurons and spinal cords. |
| Key words: edaravone free radical scavenger spinal nerve liagation neuropathic pain pJNK |
