| 摘要: |
| 目的:筛选大鼠晕船行为学指标，探讨大鼠晕船适应时间及规律。方法:运用Crampton旋转刺激模型，对SD大鼠进行重复刺激，实时观察晕船刺激过程中尿液、粪便排泄量及刺激后自发活动的变化；采用抗晕药物干预法检验各行为学指标的特异性。结果:与正常对照组相比，晕船刺激第1天刺激组大鼠粪便量显著增加(P<0.05)，自发活动显著减少（P<0.01）；随着刺激天数的增加，刺激组粪便量、自发活动均逐渐恢复，且与刺激天数间存在一定相关性（P<0.01），并分别于第9天和第7天下降至正常对照水平；用药组以东莨菪碱（0.5、1 mg/kg）灌胃后再进行旋转刺激，各行为学指标与正常对照组相比均无统计学差异。结论:旋转刺激时的排便量和刺激后的自发活动量可有效判断大鼠晕动病，连续刺激9 d左右后大鼠可达到晕船适应。 |
| 关键词: 晕动病 适应 动物模型 神经行为学表现 |
| DOI：10.3724/SP.J.1008.2009.01119 |
| 投稿时间：2008-12-12修订日期：2009-07-15 |
| 基金项目: |
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| Screen of seasickness behavior indices of simulated seasickness in rats and observation of adaptability rules |
| WANG Jun-qin△,CAI Yi-ling△,CHEN Xin-ming,TAO Dong-yi,FU Ze-jian,WANG Xiao-li,GUO Jun-sheng* |
| (Department of Military Hygiene,Faculty of Navy Medicine,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To screen for seasickness behavior parameters in rats with simulated seasickness,so as to investigate the rule of seasickness adaptation in rats.Methods:SD rats were stimulated daily by Crampton model.Urine volume (UV) and amount of fecal particle (FP) were observed during stimulation,and the spontaneous activity (SA) was also determined immediately after stimulation.Interventional experiments were performed using anti-motion sickness drugs to examine the specificity of the indices.Results:On the first day of stimulation,the FP of stimulating group increased significantly compared with the normal control group (P<0.05),while SA decreased significantly (P<0.01).FP and SA of stimulating group gradually recovered with the increase of stimulation period; there was a significant correlation between FP,SA with stimulation times (P<0.01).FP and SA recovered to the normal levels on the 9th,7th day,respectively.The experimental group received gastric perfusion of scopolamine（0.5,1 mg/kg）and was then subjected to rotation stimulation.The indices had no significant difference between the experimental group and the normal control group.Conclusion:It is indicated that FP during stimulation and SA after stimulation can be used for effective judgment of seasickness in rats.The rats become adapted to seasickness after 9 days’ continuous stimulation. |
| Key words: motion sickness adaptation animal models neurobehavioral manifestations |
