| 摘要: |
| 目的:观察原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者血清中Th17分化相关细胞因子（IL-1β、IL-6、IL-17、IL-23、TGF-β）的变化,探讨Th17细胞在PBC发病过程中的作用。方法:采用酶联免疫吸附试验（ELISA）方法比较PBC患者（n=78）、乙型肝炎后肝硬化患者（PHC,n=30）,健康对照（HC,n=60）血清中Th17分化相关细胞因子含量。结果:与HC组相比,PBC患者血清IL-1β、IL-6、IL-17A和IL-23水平显著升高（P<0.05）,而TGF-β水平差异无统计学意义;与PHC组相比,PBC患者血清IL-1β、IL-6和TGF-β水平显著降低,而IL-17和IL-23水平显著升高（P<0.05）。结论:PBC患者处于慢性炎症状态,Th17细胞可能在PBC的发病和发展过程中发挥着重要的作用。 |
| 关键词: 原发性胆汁性肝硬化 Th17细胞 细胞因子 |
| DOI：10.3724/SP.J.1008.2009.0703 |
| 投稿时间：2009-01-06修订日期：2009-04-20 |
| 基金项目:国家高科技研究发展计划（“863”计划,2006AA02Z496）,上海市科学技术委员会优秀学科带头人基金（07XD14013）. |
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| Serum profile of Th17-related cytokines in patients with primary biliary cirrhosis |
| ZHOU Yun-heng,RONG Guang-hua,XIONG Yi-song,ZHU Ye,GENG Hong-lian,YANG Zai-xing,ZHONG Ren-qian* |
| (Department of Laboratory Medicine,Key Laboratory and Clinical Immunology Center of PLA,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China) |
| Abstract: |
| Objective:To observe the serum profile of Th17-related cytokines（IL-1β,IL-6,IL-17A,IL-23, and TGF-β）in patients with primary biliary cirrhosis,so as to study the role of Th17 cells in the pathogenesis of primary biliary cirrhosis (PBC).Methods:Seventy-eight patients with PBC,30 patients with HBV-related posthepatitic cirrhosis(PHC),and 60 healthy controls (HC) were enrolled in this study.The serum levels of above five Th17-related cytokines were determined by enzyme-linked immunosorbent assay (ELISA).Results:The levels of IL-1β,IL-6,IL-17A, and IL-23 in PBC group were significantly higher than those in HC group (P<0.05); the levels of TGF-β were comparable between the PBC group and HC group.The levels of IL-1β,IL-6, and TGF-β were significantly lower and IL-17A,IL-23 were significantly higher in the PBC group compared with those in the PHC group (P<0.05).Conclusion:Our findings indicate that PBC patients are in a status of chronic inflammation.Th17 subset may play an important role in the development and progression of PBC. |
| Key words: primary biliary cirrhosis Th17 cells cytokines |
