| 摘要: |
| 目的:观察创伤弧菌致死性感染对小鼠血液系统的影响及主要脏器的病理变化，探讨其可能的致死机制。方法:建立创伤弧菌致死性感染小鼠模型，ELISA法检测小鼠血清TNF-α、IL-1β、TF水平；采用全自动血液生化分析仪测定小鼠血清总胆红素、肌酐、尿素等生化指标含量；采用自动化血细胞分析仪进行全血细胞分析；创伤弧菌感染后8 h，取小鼠心脏、肺、肝脏、脾脏、肾脏，光镜、电镜下观察器官病理改变。结果:创伤弧菌感染后小鼠血清TNF-α、IL-1β、TF水平显著升高(P<0.05)；血清尿素、肌酐、总胆红素、淀粉酶及丙氨酸转氨酶、尿酸、天冬氨酸转氨酶表达均显著升高(P<0.05)；感染后白细胞计数、血小板计数、淋巴细胞百分比下降显著(P<0.05)，红细胞计数、单核细胞百分比及血红蛋白水平显著上升(P<0.01)。感染后小鼠主要脏器均表现为充血、水肿、组织结构破坏、炎症细胞浸润，电镜下可见肝细胞凋亡。结论:创伤弧菌致死性感染可能通过引发机体过度炎症反应，激活凝血系统，诱发机体广泛组织损伤，导致不可逆性多器官功能衰竭，最终引起死亡。 |
| 关键词: 创伤弧菌 炎症反应 多器官功能衰竭 致死机制 |
| DOI：10.3724/SP.J.1008.2009.01122 |
| 投稿时间：2009-01-20修订日期：2009-10-11 |
| 基金项目:国家自然科学基金（30600545），国家重大新药创制科技重大专项基金（2009ZXJ09003-028），第二军医大学专项基金（05JS08）. |
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| Effect of lethal Vibrio vulnificus infection on blood system and pathology changes of major organs in mice |
| RONG Huan-ling1,2,YIN Meng1,DU Da-hai3,YANG Feng1*,ZHANG Xin-rong1,ZHANG Wen-xue2 |
| (1.Department of Inorganic Chemistry,School of Pharmacy,Second Military Medical University,Shanghai 200433,China;2.Department of Anatomy and Immunology,College of Life Sciences,He’nan Normal University,Xinxiang 453007;3.Department of Clinical Diagnosis,Changhai Hospital,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective:To investigate the effect of lethal Vibrio vulnificus infection on the blood system and the pathology changes of the major organs in mice,and to explore the possible mechanism of the related death.Methods: Lethal Vibrio vulnificus-infection model was established with mice.The model mice were divided into two groups：a control group and an infection group.ELISA was used to examine the serum levels of TNF-α,IL-1β,and TF.Serum total bilirubin(TBIL),creatinine(Cre),and blood urea nitrogen (BUN) were analyzed using automatic biochemical analyzer;whole blood cell analysis was also performed.The pathological changes of the heart,lung,liver,spleen,and kidney were observed under electron and light microscopes.Results: Compared with the control group,the serum levels of TNF-α,IL-1β,and TF were significantly increased in mice after infection with Vibrio vulnificus (P<0.05); the serum levels of BUN,Cre,TBIL,diastase and alanine aminotransferase (ALT) were significantly increased (P<0.05).The ratios of WBC,platelet,and lymphocytes were all significantly decreased after infection compared with the control group (P<0.05).The ratios of red blood cells,monocytes,and Hb level were significantly increased compared with the control group (P<0.05).The pathological changes of major organs included hyperaemia,edema,inflammatory cell infiltration,and apoptosis.Conclusion: Lethal infection with Vibrio vulnificus can initiate super-inflammation reaction in mice; it can also activate the blood coagulation system and induce systemic tissue injury,finally leading to death. |
| Key words: Vibrio vulnificus super-inflammation reaction multiple organ nonfunction lethal mechanism |
