| 摘要: |
| 目的观察重组人促红细胞生成素(rhEPO)预处理对肝移植患者术后早期肝功能及核转录因子-κB(NF-κB)表达的影响,探讨rhEPO预处理对肝移植术后缺血再灌注损伤的可能作用及机制。方法26例晚期肝硬化患者随机均分为实验组和对照组(n=13),均接受肝移植治疗,实验组患者移植术前1、3、 5 d给予rhEPO 100 U/kg皮下注射,对照组相同时间点给予生理盐水(2 ml)皮下注射,供肝恢复血供后1、2、4、6 h取外周血检测肝功能,蛋白质印迹法检测外周血中NF-κB p65表达,双抗体夹心酶联免疫吸附法测定外周血TNF-α水平。结果与对照组相比,实验组移植术后各时间点外周血肝功能指标、NF-κB p65、TNF-α表达水平明显下降,差异均有统计学意义( P<0.05)。结论rhEPO预处理能抑制肝移植术后早期肝脏炎症反应,保护肝功能,可能会缓解移植后缺血再灌注损伤。 |
| 关键词: 肝移植 重组红细胞生成素 NF-κB 肿瘤坏死因子-α 再灌注损伤 丙氨酸转氨酶 天冬氨酸转氨酶 |
| DOI：10.3724/SP.J.1008.2010.084 |
| 投稿时间：2009-03-05修订日期：2009-12-09 |
| 基金项目:解放军180医院科研基金（05KY016）. |
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| Recombinant human erythropoietin preconditioning prevents expression of blood NF-κB early after liver transplantation |
| ZHANG Shao-bo,HE Qian,GE Liang,SHI Jian-she,WANG Geng-jie,ZHANG Cheng-hua* |
| (Department of Liver Transplantation,No.180 Hospital of PLA,Liver Transplantation Center of Nanjing Military Area Command,Quanzhou 362000,Fujian,China) |
| Abstract: |
| ObjectiveTo study the influence of recombinant human erythropoietin (rhEPO) preconditioning on the liver function and expression of nuclear factor-κB (NF-κB) during early stage following liver transplantation,and to investigate the possible mechanism of rhEPO preconditioning on ischemia-reperfusion injury after liver transplantation.MethodsTwenty-six patients with advanced hepatic cirrhosis were randomly divided into two groups(n=13): the rhEPo pre-treatment group received subcutaneous injection of rhEPO 100 U/kg at 1,3 and 5 d before liver transplantation,and the control group received 2 ml normal saline in the same manner. The peripheral blood samples were harvested at 1,2,4 and 6 h after blood supply recovery in the donator liver to examine the hepatic functions. The NF-κB p65 expression in the peripheral blood samples were examined by Western blotting analysis,the TNF-α level in the blood was detected by ABC enzyme linked immunosorbent assay. Serum ALT and AST were also determined.ResultsThe liver function indices and the levels of serum NF-κB p65,TNF-α in the rhEPO pre-treatment group were significantly lower than those in the control group (P<0.05).ConclusionPre-treatment with rhEPO can inhibit hepatic inflammation early after liver transplantation,protecting hepatic function and reducing ischemia-reperfusion injury after liver transplantation. |
| Key words: liver transplantation recombinant erythropoietin NF-κB tumor necrosis factor-alpha reperfusion injury alanine transaminase aspartate aminotransferase |
