| 摘要: |
| 目的:研究马来酸罗格列酮对去势大鼠骨微结构的影响。方法:21只24周龄Wistar大鼠双侧卵巢切除,随机分为对照组、罗格列酮A(RA)组、罗格列酮B(RB)组,每组7只。RA组、RB组每只大鼠分别给予马来酸罗格列酮0.35 mg/d、070 mg/d（以生理盐水溶解灌胃）,对照组以相同体积生理盐水灌胃。给药4周后使用micro-CT分别测定股骨、胫骨、椎骨的骨量/组织量(BV/TV)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)、骨小梁分离度(Tb.Sp)、骨表面积/骨量(BS/BV)。结果:与对照组相比,RA组及RB组各部位（股骨、胫骨、椎骨）Tb.N、Tb.Th和BS/BV（RA组胫骨除外）均减少(P<0.05或P<0.01)；胫骨、椎骨Tb.Sp增加（P<0.05），股骨Tb.Sp无改变（P＞0.05）；各部位BV/TV差异无统计学意义（P＞0.05）。上述检测指标在RA组与RB之间差异无统计学意义（P＞0.05）。结论:使用罗格列酮后,骨微结构受到损伤,且主要集中于松质骨。 |
| 关键词: 罗格列酮 卵巢切除术 骨 X线微型计算机断层扫描 |
| DOI：10.3724/SP.J.1008.2009.01402 |
| 投稿时间：2009-03-26修订日期：2009-11-26 |
| 基金项目:上海市科委自然基金项目(07411963100). |
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| Effect of rosiglitazone on bone microarchitecture of bilaterally ovariectomized rats |
| CHEN Sheng-guang,ZHANG Qian,SHENG Chun-jun,CHENG Xiao-yun,YANG Peng,QU Shen,WU Guo-ting,LI Wen-jun* |
| (Department of Endocrinology,the 10th People's Hospital of Shanghai,Tongji University,Shanghai 200072,China) |
| Abstract: |
| Objective:To study the effect of rosiglitazone on the bone microarchitecture of bilaterally ovariectomized(OVX) rats. Methods: Twenty-one 24-week-old skeletally mature rats were bilaterally ovariectomized,and were randomly divided into three groups (n=7):Control group(C group),Rosiglitazone A group(RA group),and Rosiglitazone B group (RB group). Rats in RA group were given i.g 0.35 mg/d rosiglitazone for four weeks and those in the RB group were given i.g 0.70 mg/d rosiglitazone. Rats in the control group were given normal saline in the same manner. The rats were sacrificed four weeks later. The bone volume/tissue volume(BV/TV),the trabecular number(Tb.N),the trabecular thickness(Tb.Th),the trabecular separation(Th.Sp),and the bone surface/bone volume(BS/BV)of the femur,tibia,and lumbar were determined by micro-computered tomography(micro-CT). Results: Compared with the control group,the Tb.N,Tb.Th,and BS/BV of the femur,tibia,and lumbar all decreased significantly in RA group (except for the BS/BV of tibia) (P<0.05,P<0.01); the Tb.SP of tibia and lumbar in the RA group were significantly increased (P<0.05); and there were no differences in BV/TV between the two groups. Compared with control group,the Tb.N,Tb.Th,and BS/BV of the femur,tibia,and lumbar were decreased significantly in RB group(P<0.05,P<0.01); the Tb.Sp of tibia and lumbar increased significantly(P<0.05); and the BV/TV had no differences between the two groups. There were no significant differences between the RA group and RB group concerning all the parameters. Conclusion: Rosiglitazone can cause injury to the bone microarchitecture,especially to the cancellous part of the bone. |
| Key words: rosiglitazone ovariectomy bone micro-CT |
