| 摘要: |
| 目的:观察慢性阻塞性肺病(COPD)大鼠肺组织局部树突状细胞（DC）的存在状况,并探讨吸入糖皮质激素布地奈德及抗胆碱能受体支气管扩张剂异丙托溴铵对COPD大鼠肺部DC数目的影响。方法:60只健康雄性SD大鼠随机分为4组（n=15）：正常对照组、COPD模型组、布地奈德组、异丙托溴铵组。后3组用两次气管内注入脂多糖(LPS,200 μg/只)及熏香烟4周的复合刺激法建立大鼠COPD模型,布地奈德组、异丙托溴铵组于第8天起每天吸入相应药液。于第31天处死,肺组织切片H-E染色观察病理改变,免疫组化染色观察DC的表达。结果:COPD模型组、布地奈德组、异丙托溴铵组均出现COPD的特征性病理改变,且肺组织内DC的数量明显高于正常对照组,差异有统计学意义（P<0.01）；布地奈德组DC数目则较COPD模型组明显减少（P<0.01）,异丙托溴铵组DC数目与COPD模型组比较差异无统计学意义。结论:DC在COPD的发病机制中有重要作用,糖皮质激素布地奈德干预可以降低COPD大鼠肺部DC的数量,异丙托溴铵则无此作用。 |
| 关键词: 慢性阻塞性肺疾病 树突状细胞 布地奈德 异丙托溴铵 |
| DOI：10.3724/SP.J.1008.2009.0706 |
| 投稿时间：2009-03-26修订日期：2009-05-29 |
| 基金项目:上海市卫生局科研基金(2006JJ073). |
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| Presence of dentritic cells in bronchi and lung tissues of rats with chronic obstructive pulmonary disease and influence of budesonide and ipratropium bromide |
| MA Zhao-hui1△,ZHANG Jing1△,CHEN Ruo-hua2,GE Xia-hui1,BAI Chong1* |
| (1.Department of Respiratory Medicine,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;2.Department of VIP Medicine,Changhai Hospital,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective:To observe the presence of dentritic cells (DC) in lung tissues of rat models of chronic obstructive pulmonary disease (COPD) and the influence of budesonide and ipratropium bromide on the number of DCs.Methods: Sixty male SD rats were randomly divided into four groups(n=15): control group,COPD model group,budesonide treatment group and ipratropium biomide treatment group.The COPD rat models were established by intratracheal instillation of lipopolysaccharide (LPS) twice and exposure to tobacco smoke for 4 weeks.Drug inhalation group received inhalation of budesonide (Pulmicort) or ipratropium bromide (Atrovent) since the second week.The rats were sacrificed at the 31th day.Lung slices were observed with H-E staining for pathological changes and the status of DC were determined by immunohistochemical method.Results: The COPD model group,budesonide treatment group, and ipratropium biomide treatment group all had the pathological changes of COPD,and their DC numbers were significantly higher than that in the control group (P<0.01).DC number in Pulmicort treatment group was significantly less than that in the COPD model group (P<0.01),and there was no significant difference between the Atrovent treatment group and the COPD model group.Conclusion: The pulmonary DCs play an important role in the pathogenesis of COPD.Pulmicort can decrease the number of DCs in rat model of COPD,and ipratropium bromide has no obvious effect on DC number. |
| Key words: chronic obstructive pulmonary disease dendritic cells budesonide ipratropium bromide |
