| 摘要: |
| 目的:自制聚乙交酯-丙交酯(poly lactide-co-glycolide acid,PLGA)神经组织工程三维支架，观察该支架对体外神经膜细胞生长的影响及其体内降解情况及炎症反应，为后续研究奠定基础。方法:通过熔融、纺丝、拉伸、编织等步骤制作编织型三维PLGA支架，扫描电镜观察内部微管道的排列规律，测量微管道孔径大小。将原代培养的神经膜细胞接种到编织型三维支架上，采用倒置相差显微镜和扫描电镜观察细胞在支架上生长、黏附、增殖和凋亡情况，并与细胞培养皿和胶原海绵培养细胞进行对照。将携带神经膜细胞的三维支架植入大鼠脊柱两侧肌肉中，H-E染色观察支架在体内的降解情况及炎症反应。结果:支架外径为3 mm，微管道直径为100 μm，均匀平行排列。三维支架与细胞培养皿中的神经膜细胞细胞黏附率、增殖率无统计学差异，与胶原海绵培养细胞差异有统计学意义（P<0.05）；三维支架与胶原海绵上的细胞凋亡率无统计学差异，二者均低于细胞培养皿培养细胞，差异有统计学意义（P<0.05）。三维支架植入体内12周后完全降解，周围无明显炎性细胞浸润。结论:自制编织型三维支架对体外神经膜细胞生长无不良影响，体内能有效降解，具有良好的生物相容性。 |
| 关键词: 生物相容性 聚乙交酯-丙交酯 组织支架 神经膜细胞 |
| DOI：10.3724/SP.J.1008.2009.01186 |
| 投稿时间：2009-04-14修订日期：2009-06-24 |
| 基金项目:国家自然科学基金 (30571887),上海市青年科技启明星计划(06QA14068). |
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| Influence of self-designed three-dimensional woven scaffolds on in vitro growth of Schwann cells and its in vivo degradation |
| YUAN Jian-dong1,FU Qiang2*,CHEN Zhi-ming2,LI Zhong-hai2,XU Hao2 |
| (1.Department of Orthopaedics,The First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China;2.Department of Orthopaedics,Changhai Hospital,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective:To prepare a self-designed three-dimensional woven scaffold using poly lactide-co-glycolide acid (PLGA),and to observe the influence of the prepared scaffold on the growth of Schwann cells in vitro and its in vivo degradation.Methods: The 3D scaffolds were prepared by means of melt spinning,extension,weaving,and other procedures.The alignment of micro-tubules was observed under the scanning electronic microscope（SEM）.The size of the micropores was also measured.Primary cultured Schwann cells were seeded on the 3D scaffolds,and the growth,adherence,proliferation,and apoptosis of Schwann cells were observed under inverted phase contrast microscopy and SEM; the results were compared between Schwann cells cultured in collagen sponge and culture dish.The scaffold carrying Schwann cells was implanted into the paraspinal muscle in rats,and H-E staining was used to observe the in vivo degradation and the inflammation responses.Results: The external diameters of the scaffold and micro-tubules were 3 mm and 100 μm,respectively,and the micro-tubules were arranged in an even and parallel manner.The adherence rates and proliferation rates of Schwann cells were similar between scaffold group and collagen sponge group,but both groups were significantly lower than the culture dish group (P<0.05).The 3D scaffold degraded completely within 12 weeks,with no visible inflammatory cells around.Conclusion: The self-designed 3D scaffold has no harmful effect on the growth of Schwann cells,and it can degrade in vivo,showing a satisfactory biocompatibility. |
| Key words: biocompatibility poly lactide-co-glycolide acid tissue scaffolds Schwann cells |
