| 摘要: |
| 目的:探讨2009年新型甲型流感病毒(A/H1N1)聚合酶PA、PB1和PB2编码基因的进化规律。方法:从NCBI流感病毒基因数据库下载2009年新型甲型H1N1流行株的PA、PB1和PB2聚合酶编码基因序列以及人、猪和禽流感病毒相应的参考序列,采用Molecular Evolutionary Genetics Analysis version 4.0（MEGA4.0）软件比对和修剪此次流行株的代表序列及所有参考株序列并构建系统树,再比对和修剪此次流行株的代表序列及人A/H1N1病毒各年代(1918~2008年)参考序列并构建系统树,同时比对此次流行株的代表序列及人A/H1N1各年代(1918~2008年)参考序列编码PB2蛋白的氨基酸序列。结果:不同地区分离的2009年新型甲型H1N1流感病毒的聚合酶PA、PB1和PB2编码基因均具有高度同源性,并聚集在一个独特的进化支上,与猪流感病毒对应基因接近。三者均与2005年美国爱荷华州分离的人A/H1N1病毒基因(A/Iowa/CEID23/2005/H1N1)具有高度的相似性。2009年新型甲型H1N1流感病毒、2005年美国爱荷华州流行的H1N1 (DQ889682)病毒PB2蛋白第627位氨基酸与禽类流感病毒相同,均为谷氨酸,而与其他人A/H1N1 (1918~2008年)病毒的赖氨酸不同。结论:2009年新型甲型H1N1流感病毒聚合酶基因可能来源于2005年美国爱荷华州分离的人A/H1N1病毒,禽流感病毒可能参与了聚合酶基因的重排过程。 |
| 关键词: H1N1甲型流感病毒进化 聚合酶基因 基因重排 |
| DOI：10.3724/SP.J.1008.2009.0632 |
| 投稿时间：2009-05-21修订日期：2009-06-05 |
| 基金项目:军队“十一五”科技攻关计划（06G65）,上海市自然科学基金（07ZR14141）,上海市公共卫生“三年行动计划”重点学科项目（08GWZX0201,08GWZX0101）. |
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| Evolutionary characteristics of polymerase PA,PB1,and PB2 genes of novel influenza virus A/H1N1 in 2009 pandemic |
| HAN Lei,YIN Jian-hua,XIE Jia-xin,LI Shu-hua,HAN Yi-fang,LU Wen-ying,SU Tong,CAO Guang-wen* |
| (Department of Epidemiology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To elucidate the evolutionary characteristics of polymerase PA,PB1,and PB2 genes of the novel influenza virus A/H1N1 in 2009 pandemic. Methods: The sequences of the PA,PB1,and PB2 genes of the novel H1N1 strains in 2009 pandemic,and the reference sequences of human,swine,and avian influenza viruses isolated during different years at different locations were retrieved from NCBI. Molecular Evolutionary Genetics Analysis version 4.0 （MEGA4.0） software was employed to align and blunt nucleotide sequences,construct phylogenetic tree,deduce and align PB2 protein sequences,and the results were compared between the novel A/H1N1 and each of the reference strains. Results: The sequences of the PA,PB1,and PB2 genes of 2009 novel A/H1N1 strains isolated from different locations shared a high homology and clustered in a unique new clade,and were close to the swine influenza viruses. The PA,PB1,and PB2 genes of the novel H1N1 viruses had a high similarity with the corresponding sequences of a human H1N1 strain isolated in Iowa State of USA in 2005 (A/Iowa/CEID23/2005/H1N1). Alignments of the deduced protein sequences showed that the 627th amino acid of PB2 of the novel H1N1 strains and A/Iowa/CEID23/2005/H1N1 were glutamic acid (Glu),which was the same as that in the avian influenza virus in Iowa State of USA in 2005 (DQ889682), and was different from those of the reference sequences of human A/H1N1 strains isolated from 1918 to 2008,which were lysine (Lys). Conclusion: The 2009 novel A/H1N1 virus might be originated from the human A/H1N1 strains isolated in 2005 in Iowa State of America (A/Iowa/CEID23/2005/H1N1),and the polymerase gene of the novel H1N1 virus might reassort with avian A influenza virus. |
| Key words: H1N1 subtype influenza A virus polymerase gene gene rearrangement |
