| 摘要: |
| 目的探讨去铁敏预处理对星型胶质细胞(AS)缺氧损伤的保护作用及可能机制。方法体外培养AS,建立去铁敏糖氧剥夺(OGD)模型,细胞分为：正常培养组、去铁敏预处理组(先给予去铁敏预处理,再给予去铁敏OGD)、OGD组(给予去铁敏OGD)。采用细胞活力测定、细胞核固缩比率、形态学改变评价去铁敏预处理后的保护效应。用免疫荧光染色检测去铁敏预处理后AS的缺氧诱导因子-1α(HIF-1α)和促红细胞生成素(EPO)蛋白表达情况,RT- PCR检测HIF-1α和EPO的mRNA变化情况。结果去铁敏预处理组细胞形态保持良好,AS活力下降减轻为58%(OGD组 25%,P<0.05),细胞核固缩百分比为38%(OGD组30%,P<0.05)。免疫荧光染色发现,体外培养的AS在预处理后出现 HIF-1α和EPO蛋白表达。RT-PCR发现去铁敏化学缺氧能上调HIF-1α及EPO mRNA表达。结论去铁敏预处理有确切有效的抗缺氧损伤作用,这种效应与保护AS有关,其机制可能是去铁敏诱导了HIF-1α和EPO表达增加。 |
| 关键词: 去铁敏 缺血预处理 缺氧诱导因子1α 红细胞生成素 |
| DOI：10.3724/SP.J.1008.2010.0144 |
| 投稿时间：2009-07-20修订日期：2009-12-14 |
| 基金项目:国家自然科学基金(30170340). |
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| Deferoxamine preconditioning protects against hypoxia injury in astrocytes |
| LI Yun-xia1,2, DING Su-ju1*, ZHAN Qing2, XIAO Lin3, GUO Wei3 |
| (1. Departmeent of Neurology, Changhai Hospital, Second Military Medical University,Shanghai 200433, China;2. Department of Neurology, Tongji Hospital,Tongji University, Shanghai 200065, China ;3. Institute of Neurology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the protective effect of deferoxamine preconditioning against hypoxia injury in astrocytes and the underlying mechanisms. MethodsAstrocytes were cultured under ischemia stress, which was mimicked by oxygen and glucose deprivation (OGD) with deferoxamine. Astrocytes were divided into three groups: normally cultured group; Deferoxamine pretreated group: astrocytes were pretreated with Deferoxamine and then treated with Deferoxamine OGD; and OGD group; astrocytes were treated with Deferoxamine OGD. The cell viability examination, the ratio of condensed nuclei, and the morphological changes were used to assess the protective effect of deferoxamine. The expression of HIF-1α and EPO protein and mRNA in astrocytes was examined by immunofluorescence staining and RT-PCR, respectively. ResultsThe morphology of AS in the deferoxamine pretreated group kept intact. AS viability in deferoxamine pretreated group was 58% of the normally cultured group, and that in the OGD group was 25% of the normally cultured group (P<0.05). The ratio of condensed nuclei in deferoxamine pretreated group was 38% and that in OGD group was 30%(P<0.05).Immunofluorescence staining found that expression HIF-1α, and EPO protein appeared in cultured astrocytes after deferoxamine pretreatment. RT-PCR confirmed that mRNA of HIF-1α and EPO was up-regulated after deferoxamine pretreatment. ConclusionDeferoxamine preconditioning can protect the astrocytes from hypoxia damage, which is related to deferoxamine-induced increase of HIF-1α and EPO expression. |
| Key words: deferoxamine ischemic preconditioning hypoxia-inducible factor 1 alpha erythropoietin |
