| 摘要: |
| 目的 观察重组Galectin9腺病毒对小鼠心脏移植物生存期及调节性T(Treg)细胞分化和增殖的影响。方法 建立小鼠心脏移植模型，随机均分为3组(n=10)：对照组(术后用0.9%NaCl溶液)、空腺病毒组、重组Galectin9腺病毒组，记录心脏移植物生存时间，于移植心停搏或术后14 d取脾，分离单个核细胞，流式细胞仪检测CD4+CD25+Treg细胞比例。ELISA法检测外周血IL10、TGFβ1浓度。结果 与对照组、空腺病毒组相比，重组Galectin9腺病毒组心脏移植物生存时间明显升高(P<0.01)，重组Galectin9腺病毒组CD4+CD25+Treg细胞比例明显升高(P<0.01)。重组Galectin9腺病毒组IL10、TGFβ1的含量明显高于对照组及空腺病毒组(P<0.01)。结论 重组Galectin9腺病毒能促进体内CD4+CD25+Treg细胞的分化与增殖，明显提高小鼠心脏移植物的生存时间。 |
| 关键词: Galectin-9 调节性T淋巴细胞 心脏移植 细胞分化 细胞增殖 |
| DOI：10.3724/SP.J.1008.2010.0595 |
| 投稿时间：2010-01-06修订日期：2010-04-08 |
| 基金项目:国家自然科学基金面上项目(30772056). |
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| Influence of adenovirus carrying Galectin 9 gene on differentiation and proliferation of CD4+CD25+ regulatory T cells in mice with heart transplantation |
| YANG Jie-yu, GUO Wen-yuan, XIAO Liang, FU Hong, SHI Xiao-min, LIU Fang, DING Guo-shan*, FU Zhi-ren |
| (Department of Organ Transplantation, Changzheng Hospital, Second Military Medical University,Shanghai 200003, China) |
| Abstract: |
| Objective To explore the influence of adenovirus carrying Galectin9 gene on the survival of cardiac transplant and the differentiation and proliferation of CD4+CD25+Treg cells in mice receiving heterotopic heart transplantation.Methods Neck heterotopic heart transplantation models were established with male BALB/c→C57BL/6 mice by Cuff method, and they were randomly divided into 3 groups (n=10): control group (received 0.9% sodium chloride solution after operation), adenovirus group, and pAdeasyTM Galectin9 treatment group. The survival time of the graft hearts was recorded. The spleens of mice were collected when the graft hearts stopped beating or 14 days after operation; the mononuclear cells were isolated and the proportion of CD4+CD25+ Treg cells was determined by flow cytometry. Treg cell related cytokines (IL10, TGFβ1) in the peripheral blood were assayed by ELISA.Results The cardiac allografts in pAdeasyTM Galectin9 group survived significantly longer than those in the other two groups (P<0.01), and the proportion of CD4+CD25+ Treg cells in CD4 + T cells was significantly higher(P<0.01). The levels of IL10 and TGFβ1 were increased in pAdeasyTM Galectin9 group compared with control group and adenovirus group(P<0.01).Conclusion pAdeasyTM Galectin9 can promote the proliferation and differentiation of CD4+CD25+ Treg cells in vivo, and can greatly improve the survival time of cardiac allograft after heart transplantation in mice. |
| Key words: Galectin-9 regulatory T lymphocytes heart transplantation cell differentiation cell proliferation |
