| 摘要: |
| 目的 分析小鼠血共刺激分子CD86和各淋巴细胞亚群活化分子CD69的表达,探讨Toll样受体4(TLR4)在脂多糖(LPS)诱发的早产中的作用。方法 在预先阻断TLR4或不阻断TLR4的条件下,腹腔注射LPS建立小鼠炎症诱导性早产模型,计算各组(LPS组、抗TLR4组和对照组)的早产率和死胎率。应用流式细胞仪检测血CD45+CD86+、CD3+CD69+、CD19+CD69+和CD49b+CD69+细胞的百分率。结果 LPS组的早产率\[50.0%(8/16)\]和死胎率\[11.0%(9/82)\]均高于对照组\[0(0/16),3.1%(5/163),P<0.01或P<0.05\],抗TLR4组的早产率\[6.3%(1/16)\]和死胎率\[3.9%(6/154)\]均低于LPS组(P<0.01或P<0.05)。预先阻断TLR4明显抑制LPS所致的血CD45+CD86+、CD3+CD69+和CD49b+CD69+细胞水平的升高(P均<0.01)。结论 LPS与其特异性受体TLR4作用,触发CD86+树突状细胞的活化和动员,导致T细胞和NK细胞等淋巴细胞亚群的激活,在早产的发生中具有重要作用。 |
| 关键词: 早产 脂多糖类 Toll样受体4； CD86； CD69； 淋巴细胞 |
| DOI：10.3724/SP.J.1008.2010.0955 |
| 投稿时间：2010-02-12修订日期：2010-08-25 |
| 基金项目:广东省自然科学基金博士启动项目(7301628), 广东省医学科研基金(A2008512), 广州市医药卫生科技项目(2007-YB-025). |
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| Analysis of CD86 and CD69 expression on blood lymphocytes in inflammation-induced premature mice |
| LI Li-ping*, KANG Jia-li, FENG Shu-yu, LEI Wei-hua |
| (Department of Obstetrics and Gynecology, First People’s Hospital of Guangzhou, Guangzhou 510180, Guangdong, China) |
| Abstract: |
| Objective To investigate the role of Toll-like receptor 4 (TLR4) in lipopolysaccharide (LPS)-induced preterm delivery by analyzing the CD86 and CD69 expression in lymphocyte subgroups of mice. Methods LPS was administered intraperitoneally to establish a mouse model of preterm delivery, with or without TLR4 blockade. The incidences of preterm delivery and fetal death were calculated in each group (LPS group, TLR4 blockade group, and control group). The percentages of blood CD45+CD86+, CD3+CD69+, CD19+CD69+ and CD49b+CD69+ subsets were measured by flow cytometry. Results The incidences of preterm delivery and fetal death in LPS group were significantly higher than those in the control group (50.0% \[8/16\] vs 0\[0/16\]; 11.0%\[9/82\] vs 3.1%\[5/163\], P<0.01 or 0.05). The incidences of preterm delivery(6.3%\[1/16\]) and fetal death (3.9%\[6/154\]) in the TLR4 blockade group were significantly lower than those in the LPS group (P<0.01 or 0.05). TLR4 blockade almost completely abrogated LPS-induced increase of CD45+CD86+, CD3+CD69+ and CD49b+CD69+ cell proportions (P<0.01). Conclusion Interaction between LPS and its receptor TLR4 triggers the mobilization of CD86+ dendritic cells, which subsequently activates blood T cells and NK cells and plays an important role in preterm delivery. |
| Key words: premature birth lipopolysaccharides Toll-like receptor 4 CD86 CD69 lymphocytes |
