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| 脐带间充质干细胞对C57BL/6小鼠Lewis肺癌生长及转移的影响 |
| 卢兆桐1*,李福泉1,2,邹志强1,袁耒1,王玉波1 |
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(1.济南军区总医院胸外科,济南250031
2.东平人民医院胸外科,泰安 271500
*通信作者) |
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| 摘要: |
| 目的 利用Lewis肺癌动物模型探讨脐带来源的间充质干细胞(MSC)对肺癌生长及转移的影响。方法 用16只C57BL/6小鼠分别建立Lewis肺癌模型，并随机分为对照组(NS组)和MSC组，每组8只。MSC组分别于接瘤后第7、12、17天时，尾静脉注射脐带MSC(1×106/只)，在第21天时处死全部荷瘤小鼠，观察瘤体生长情况及肺转移情况。结果 NS 组和MSC组的平均瘤体质量分别为(4.587 5±1.04) g、 (4.155±1.13) g，两组相比差异无统计学意义(P=0.59)。NS组和MSC组的平均肺转移数分别为(3.75±1.39)个、(1.13±1.13)个，两组相比差异有统计学意义(P<0.01)；抑制转移率为70.0%。结论 脐带来源的MSC对C57BL/6小鼠Lewis肺癌本身的生长无影响，但能够抑制肿瘤的转移。 |
| 关键词: Lewis肺癌 脐血干细胞移植 间质干细胞 肿瘤转移 |
| DOI：10.3724/SP.J.1008.2012.00355 |
| 投稿时间：2011-07-10修订日期：2012-03-10 |
| 基金项目: |
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| Effects of umbilical cord-derived mesenchymal stem cells on growth and metastasis of Lewis lung carcinoma in C57BL/6 mice |
| LU Zhao-tong1*,LI Fu-quan1,2,ZOU Zhi-qiang1,YUAN Lei1,WANG Yu-bo1 |
(1. Department of Cardiothoracic Surgery, General Hospital, PLA Jinan Military Area Command, Jinan250031, Shandong, China
2. Department of Cardiothoracic Surgery, People’s Hospital of Dongping, Taian 271500, Shandong, China
*Corresponding author.) |
| Abstract: |
| Objective To study the effects of umbilical cord-derived mesenchymal stem cells (MSCs) on the growth and metastasis of lung cancer using Lewis lung carcinoma animal models. Methods Animal models of Lewis lung carcinoma were established in 16 C57BL/6 mice and were randomly divided into normal saline (NS) group and MSC group (n=8). Mice in the MSC group were injected with 1×106 MSCs via the tail vein at day 7, 12, and 17. All mice were sacrificed to observe the lung metastases and the tumor size at day 21. Results The average tumor weight was (4.587 5±1.04) g in the NS group and (4.155±1.13) g in the MSC group (P=0.59). The average number of lung metastasis nodules was 3.75±1.39 in the NS group and 1.13±1.13 in the MSC group (P<0.01), showing an inhibitory rate of 70%. Conclusion Umbilical cord-derived MSCs have no effects on the growth of Lewis lung carcinoma in mice, but they can inhibit tumor metastasis. |
| Key words: Lewis lung carcinoma cord blood stem cell transplantation mesenchymal stem cells neoplasm metastasis |
