| 摘要: |
| 目的 应用高效液相色谱法对龙爪槐角与槐角对照药材中主要的黄酮苷元成分进行比较, 探索龙爪槐角替代国槐槐角应用的可行性。方法 采摘石家庄不同地点的龙爪槐角, 以槐角对照药材为参照, 提取水解后, 高效液相色谱法绘制HPLC色谱图, 同时测定其中的主要黄酮苷元成分槲皮素、山柰酚和主要异黄酮苷元染料木素的含量。HPLC测定条件: Diamonsil C18柱(150 mm×4.6 mm, 5 μm), 0.4%磷酸-甲醇(50∶50)为流动相, 流速1.0 ml/min, 检测波长360 nm。结果 龙爪槐角与槐角对照药材HPLC色谱图峰型基本一致, 其中的槲皮素、染料木素和山柰酚平均含量分别为(0.30±0.07)%、(3.04±0.27)%和(2.74±0.30)%, 与槐角对照药材中三者的含量接近。结论 龙爪槐角与槐角对照药材的主要黄酮苷元成分种类相同, 含量接近, 具有代替槐角的应用前景。 |
| 关键词: 龙爪槐角 槐角 槲皮素 染料木素 山柰酚 高压液相色谱法 |
| DOI：10.3724/SP.J.1008.2010.00 |
| 投稿时间：2010-05-19修订日期：2010-07-28 |
| 基金项目:全军医学科研“十一五”计划面上项目(06MB335). |
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| Three flavonoid aglycone equivalents in fruits of Sophora japonica var. pendula Loud. and authentic Fructus sophorae: a comparative study |
| LIU Jing-dong*, WANG Zhi-ling, GOU Ling-yan, WANG Jing, LIU Hui-min, WANG Xiu-qing |
| (Center of Apparatus, Bethune Military Medical College, the Forth Military Medical University, Shijiazhuang 050081, Hebei, China) |
| Abstract: |
| Objective To compare the chromatographic profiles and contents of three flavonoid aglycone equivalents (quercetin, genistein, and kaempferol) in the fruits of Sophora japonica var. pendula Loud. and the authentic Fructus sophorae, so as to provide a theoretical basis for using the fruit of Sophora japonica var. pendula Loud. as a substitute for Fructus sophorae. Methods The fruits of Sophora japonica var. pendula Loud. were collected from different areas of Shijiazhuang in Winter. The flavonoids in the fruits were extracted by successive extraction with methanol. The acidic-hydrolyzed extracts were separated on a Diamonsil C18 column (150 mm×4.6 mm, 5 μm). 0.4% phosphoric acid-methanol (5050, V/V) was used as mobile phase. The flow rate of mobile phase was 1.0 ml/min and the effluents were monitored at 360 nm. Results The chromatographic profiles of the two fruit extracts were consistent basically. The contents of quercetin, genistein, and kaempferol in the fruits of Sophora japonica var. pendula Loud. were (0.30±0.07)%, (3.04±0.27)%, and (2.74±0.30)%, respectively, which were similar to those in the authentic Fructus sophorae. Conclusion It is found that the contents of major flavonoid aglycones in the fruit of Sophora japonica var. pendula Loud. are similar to those in the authentic Fructus sophorae, making it a potential substitute for the Fructus sophorae. |
| Key words: Sophora japonica var. pendula Loud. Fructus sophorae quercetin genistein kaempferol high pressure liquid chromatography |
