| 摘要: |
| 目的 探讨出生后肝脏甲胎蛋白(AFP)基因异常高表达在ZBTB20基因敲除小鼠生长发育障碍、严重低血糖和未成年死亡等表型中可能的病理作用。方法 将ZBTB20单基因敲除小鼠与AFP单基因敲除小鼠杂交，建立ZBTB20与AFP的联合基因敲除小鼠模型，观察并监测其与ZBTB20单基因敲除小鼠在生长发育、存活率及血糖水平上的差异。结果 ZBTB20/AFP双基因敲除小鼠繁殖成功后，通过观察和监测发现其在生长发育、生存率及血糖代谢等方面与ZBTB20单基因敲除小鼠相比无明显差异。结论 ZBTB20单基因敲除小鼠生长发育障碍、严重低血糖和未成年死亡等表型与其AFP基因出生后异常开放无显著关系。 |
| 关键词: 甲胎蛋白类 ZBTB20 锌指蛋白 基因敲除小鼠 |
| DOI：10.3724/SP.J.1008.2010.01045 |
| 投稿时间：2010-08-16修订日期：2010-09-28 |
| 基金项目:国家自然科学基金(90608026,30772478). |
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| Pathological implication of postnatally up-regulated AFP expression in ZBTB20 knockout |
| ZHANG Hai1, XIE Zhi-fang1, Claude Szpirer2,,ZHANG Wei-ping1* |
| (1. Department of Pathophysiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China;2. Universit Libre de Bruxelles, Institut de Biologie et de M decine Mol culaires, 12 Rue Profs Jeneer & Brachet, B-6041 Gosselies, Belgium) |
| Abstract: |
| Objective To investigate the pathological roles of postnatally up-regulated alpha-fetoprotein(AFP) expression in ZBTB20 knockout mice in postnatal growth retardation, metabolic dysfunction, and pre-mature mortality. Methods ZBTB20 and AFP knockout mice were generated by crossbreeding ZBTB20 knockout mice with AFP knockout mice.The postnatal growth, survival rate, and blood glucose of ZBTB20/AFP double knockout mice were observed and compared with those of ZBTB20 knockout mice. Results There were no significant differences in postnatal growth, survival, and glucose homeostasis between ZBTB20/AFP doube knockout mice and ZBTB20 knockout mice. Conclusion The postnatally up-regulated liver AFP in ZBTB20 knockout mice is not associated with the growth retardation, hypoglycemia, or pre-mature mortality. |
| Key words: alpha-fetoproteins ZBTB20 zinc finger protein knockout mice |
