| 摘要: |
| 目的探讨雾化吸入肺表面活性物质(PS)对油酸型急性肺损伤(ALI)大鼠血液氧合水平的影响。方法43只SD大鼠随机分为假手术组、模型组、雾化生理盐水组和雾化PS组。颈外静脉注入油酸(0.2 ml/kg)复制ALI模型,约1 h后雾化生理盐水组和雾化PS组分别雾化吸入生理盐水和160 mg PS。实验过程中测定大鼠血气分析指标和呼吸频率(BR)。计算大鼠存活率。结果雾化吸入PS后,大鼠动脉血氧分压(PaO2)及动脉血氧饱和度(SaO2)均逐渐增加,至雾化吸入4 h后,PaO2及SaO2分别增加至99.4 mmHg(1 mmHg=0.133 kPa)及98.2%,与雾化生理盐水组相比,差异有统计学意义(P<0.05)；大鼠BR自雾化吸入PS 0.5 h后即开始下降,雾化吸入PS 4 h后,BR降至100次/min,低于同时间点的雾化生理盐水组(P<0.05)。雾化PS组大鼠的存活率为100%,与雾化生理盐水组(60%)相比,差异有统计学意义(P<0.05)。结论雾化吸入PS能明显提高ALI大鼠血液的氧合状况,改善大鼠的肺功能及提高其存活率。 |
| 关键词: 气雾剂 肺表面活性物质 急性肺损伤 血气分析 |
| DOI：10.3724/SP.J.1008.2011.01 |
| 投稿时间：2010-09-12修订日期：2010-12-24 |
| 基金项目:军队“十一五”科技攻关项目(06G63)，上海市科委重点基础项目(03JC14002). |
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| Effect of aerosolized pulmonary surfactant on blood oxygenation in rats with acute lung injury |
| YAN Ya-min1,LI Yi-dong2,SONG Xing-lei2,WANG Yan2,LU Jian2*,WANG Zhi-hong1* |
| (1. Department of Clinical Nursing, School of Nursing, Second Military Medical University, Shanghai 200433, China;;2. Department of Pathophysiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the effect of aerosolized pulmonary surfactant (PS) on the blood oxygenation in rats with acute lung injury(ALI). MethodsFortythree SD rats were randomly divided into 4 groups: sham group (operated only, n=10), model group(n=10), airNS group (inhaling aerosolized normal saline, n=10), and airPS group (inhaling aerosolized PS\[160 mg\], n=13). ALI rats were induced by oleic acid(0.2 ml/kg) through jugular vein. The arterial blood gas and breathing rates were determined during the experiment in all groups. The survival rates of all groups were determined after experiments. ResultsPS aerosolization led to a gradual increase of PaO2 and SaO2. Four hours after inhalation of aerosolized PS, the PaO2 and SaO2 increased to 99.4 mmHg(1 mmHg=0.133 kPa) and 98.2%, respectively, which were significantly higher than those in the airNS group (P<0.05). The breathing rate began to decease 0.5 h after inhalation of aerosolized PS and decreased to 100 breaths/min 4 h later, which was significantly lower than that in the airNS group at the same time point (P<0.05). The survival rate of animals in the airPS group was 100%, also significantly higher than that in the airNS group (60%, P<0.05). ConclusionAerosolized PS through spontaneous breathing can improve the blood oxygenation, pulmonary function and survival rates of ALI rats. |
| Key words: aerosols pulmonary surfactant acute lung injury blood gas analysis |
