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| IL-22在支气管哮喘患者血清中的表达及其受体IL-22R1在人气道细胞中的表达 |
| 张旃1,罗雅玲1*,周丽丽2,赖文岩3,徐建4,黎振兴1,任敦强1,叶涵1,钟浩海1 |
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| (2.南方医科大学附属南方医院肾内科,广州 510515;3.南方医科大学附属南方医院心血管内科重点实验室,广州 510515;4.南方医科大学附属南方医院肾移植科,广州 510515) |
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| 摘要: |
| \[摘要\]目的检测IL-22在支气管哮喘患者血清中的表达,检测IL-22R1在人气道上皮细胞、气道平滑肌细胞、肺成纤维细胞中的表达,寻找IL-22作用的靶细胞。方法应用ELISA法检测36例支气管哮喘患者及20例正常对照者血清IL-22及IL-17的表达,同时检测36例患者肺通气功能,根据1秒率(FEV1/FVC)及FEV1占预计值的百分比将支气管哮喘患者分为支气管激发试验阳性组(17例)和支气管舒张试验阳性组(19例)。应用实时定量PCR检测人气道平滑肌细胞、人肺成纤维细胞、人气道上皮细胞IL-22R1 mRNA的表达,用免疫荧光以及蛋白质印迹法检测IL-22R1蛋白在上述3种细胞中的表达。结果支气管哮喘患者血清IL-22及IL-17水平与正常对照组相比差异无统计学意义,但支气管舒张试验阳性组患者血清IL-22和IL-17水平高于支气管激发试验阳性组(P<0.05)。IL-22R1 mRNA及蛋白在上述3种细胞均有表达。结论IL-22可能涉及支气管哮喘的发生发展过程,气道平滑肌细胞、肺成纤维细胞、人气道上皮细胞可能均是IL-22发挥作用的靶细胞。 |
| 关键词: IL-22 IL-22R1 气道上皮细胞 气道平滑肌细胞 肺成纤维细胞 |
| DOI：10.3724/SP.J.1008.2010.01291 |
| 投稿时间：2010-09-16修订日期：2010-09-29 |
| 基金项目: |
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| Serum IL-22 level in asthmatic patients and expression of IL-22R1 in human airway cells |
| ZHANG Zhan1, LUO Ya-ling1*, ZHOU Li-li2, LAI Wen-yan3, XU Jian4, LI Zhen-xing1, REN Dun-qiang1, YE Han1, ZHONG Hao-hai1 |
| (1. Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China;2. Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China;3. Laboratory of Cardiovascular Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China;4. Department of Kidney Transplantation, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China) |
| Abstract: |
| \[Abstract\]ObjectiveTo examine the serum level of IL-22 in asthmatic patients and the expression of IL-22R1 in human airway epithelial cells, human airway smooth muscle cells, and lung fibroblasts, so as to explore the target cells of IL-22. MethodsThe serum levels of IL-22 and IL-17 in 36 asthmatic patients and 20 normal control subjects were measured by enzyme-linked immunosorbent assay (ELISA). And lung function of the asthmatic patients was assessed by Gaeger spirometry. According to the values of FEV1/FVC and FEV1%, the patients were divided into two groups: bronchodilation test positive group (19 patients) and bronchial provocation test positive group (17 patients). The expression of IL-22R1 mRNA in human airway epithelial cells, human airway smooth muscle cells, and lung fibroblasts was examined using real-time PCR, and IL-22R1 protein expression was detected by immunofluorescence staining and Western blotting analysis. ResultsThere was no significant difference in serum IL-22 and IL-17 levels between the asthmatic patients and normal controls. The serum IL-22 and IL-17 levels in asthmatic patients positive for bronchodilation test was significantly higher than those positive for bronchial provocation test(P<0.05). IL-22R1 mRNA and protein were detected in all the 3 types of cells. ConclusionIL-22 may be involved in the pathogenesis of asthma, and human airway epithelial cells, human airway smooth muscle cells, and lung fibroblasts may all be the target cells of IL-22. |
| Key words: IL-22 IL-22R1 airway epithelial cell airway smooth muscle cell lung fibroblasts |
