| 摘要: |
| \[摘要\] 目的 探讨人类巨细胞病毒感染对脐动脉血管平滑肌细胞内胆固醇代谢的影响及机制。方法 自脐动脉分离平滑肌细胞,以1个感染复数（multiplicity of infection,MOI）的人类巨细胞病毒攻击,同时用含3%小牛血清的DMEM/F12维持液进行模拟感染以用作对照。测定病毒攻击后平滑肌细胞内胆固醇含量的变化,以基因表达谱芯片技术检测病毒攻击后细胞基因表达谱的变化,研究与胆固醇代谢有关酶的变化并经荧光定量RTPCR验证。结果 细胞内胆固醇含量在病毒感染后72 h\[（0.71±0.06） μmol/106 cells\]显著高于模拟感染组（P=0.000 2）。脐动脉平滑肌细胞被人类巨细胞病毒攻击后48、72 h,细胞中与胆固醇合成代谢有关的HMGCoA合成酶及HMGCoA还原酶的表达明显上调(P<0.01)。结论 人类巨细胞病毒感染可能通过上调HMGCoA合成酶及HMGCoA还原酶基因的表达水平,导致血管平滑肌细胞内胆固醇合成增加,引起细胞内胆固醇代谢失衡。 |
| 关键词: 巨细胞病毒 HMG辅酶A合成酶 HMG辅酶A还原酶 胆固醇 脐动脉 平滑肌细胞 |
| DOI：10.3724/SP.J.1008.2011.0150 |
| 投稿时间：2010-10-18修订日期：2011-01-20 |
| 基金项目:湖南省卫生厅科研基金(B2008 079). |
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| Influence of human cytomegalovirus infection on cholesterol metabolism in smooth muscle cells of umbilical arteries and the related mechanisms |
| LI Ling-fang1,CHEN Hua2,WU Guo-jun1* |
| (1. Xiangya School of Medicine,Central South University,Changsha 410078,Hunan,China; 2. Department of Clinical Laboratory, The Third Hospital of Changsha,Changsha 410015,Hunan,China) |
| Abstract: |
| \[Abstract\] Objective To investigate the effect of human cytomegalovirus(HCMV) infection on cholesterol metabolism in smooth muscle cells of the umbilical arteries and the related mechanismsMethods Vascular smooth muscle cells were isolated from umbilical arteries and were challenged with 1 MOI HCMV, and the intercellular content of cholesterol was determined.Cells treated with DMEM/F12 solution containing 3% fetal bovine serum were taken as mock infection controls.The differentially expressed genes were screened by cDNA microarray in each group,and the abnormally expressed genes associated with cholesterol metabolism were identified by fluorescence quantitative RTPCR.Results The intracellular content of cholesterol (\[0.71±0.06\] μmol/106 cells) was significantly higher in HCMV infection group at 72 h after infection compared with that in the control group (t=7.950,P=0.000 2).cDNA microarray and fluorescence quantitative RTPCR showed that the expressions of HMGCoA synthetase and HMGCoA reductase were greatly upregulated compared with those in the mock infection group 48 and 72 h after infection (P<0.01).Conclusion Human cytomegalovirus infection can increase the synthesis of cholesterol through upregulating cholesterol metabolismassociated genes in vascular smooth muscle cells,leading to unbalancd cholesterol metabolism. |
| Key words: cytomegalovirus HMG-CoA synthetase HMG-CoA reductases cholesterol umbilical arteries smooth muscle cells |
