| 摘要: |
| 目的探讨缺血再灌注(IR)损伤对心肌细胞自噬相关基因(autophagy-related gene,Atg)表达的影响。方法成年雄性SD大鼠随机分为2组:对照组(假手术组),于左前降支(left anterior descending, LAD)下方穿过5-0丝线,不结扎;缺血再灌注损伤组:结扎LAD,45 min后松结扎线再灌注120 min。利用RT-PCR方法,检测各组心肌Beclin 1和Atg5 mRNA表达；应用蛋白质印迹分析法检测心肌LC3蛋白表达变化。结果缺血再灌注损伤后心肌Beclin |
| 关键词: 心肌再灌注损伤 心肌细胞 自噬 |
| DOI：10.3724/SP.J.1008.2011.0776 |
| 投稿时间：2010-12-07修订日期：2011-06-22 |
| 基金项目:国家自然科学基金青年科学基金项目 |
|
| Ischemia-reperfusion injury induced overexpression of autophagy-related genes in cardiomyocytes |
| XIAO Jian1,HE Bin3,ZHU Xiao-yan2,ZHANG Yu-feng1,KANG Bo1,WANG Zhi-nong1* |
| (1. Department of Cardiothoracic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;3. Department of Anaesthesiology, Xinhua Hospital, Shanghai Jiaotong University, Shanghai 200092, China;2. Department of Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective:To compare the autophagy-related gene stimulated by ischemical reperfusion (IR) injury. Methods: The SD rats (250-300 g) were divided
randomly into 2 group：A: (Control group) the rat underwent thoracotomy without left anterior descending coronary (LAD) ligated；B: (Ischemia-Reperfusion Injury group the left anterior descending coronary was ligated for 45min, followed by reperfusion for 2 h. The mRNA expression levels of Beclin1 and Atg5 were examined with RT-PCR method, and the protein of LC3 was determined with Western-blot method. Results: The relative amount of the mRNA expression level of Beclin1and Atg5 was enhanced by IR to 2.45 and 1.6 times, respectively. The LC3-Ⅱprotein increased, which increased the ratio of LC3-Ⅱ/LC3-Ⅰ（IR group, 1.4; Con group, 0.2）. Conclusion: Ischemia-Reperfusion Injury may induce cardiomyocytes autophagy, which contribute to cell death, by increasing the expression of the Atg family. |
| Key words: Ischemia-Reperfusion Injury,myocardiocyte,autophagy |
